{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Nemati S"],"funding":["National University Ireland, Galway","Research Ireland"],"pagination":["129-143"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12325430"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["401(2)"],"pubmed_abstract":["Macromolecular crowding (MMC) is a biophysical phenomenon that has proven effective in enhancing extracellular matrix (ECM) deposition in vitro. However, MMCs potential in neuroglial cell cultures remains underexplored. This study investigates the effects of three distinct MMC agents [carrageenan (CR), dextran sulphate (DxS) and FicollⓇ cocktail (FC)] on ECM deposition and cell behaviour of Neu7 and primary astrocytes. While the viability and metabolic activity of Neu7 astrocytes were unaffected by any of the crowding agents, primary astrocytes exhibited a significant decrease in viability and metabolic activity in the presence of CR and DxS. The addition of CR, DxS, and FC resulted in a significant increase in deposition of fibronectin, collagen IV, collagen I, GFAP and CS56 in Neu7 astro"],"journal":["Cell and tissue research"],"pubmed_title":["The effect of macromolecular crowders on deposition of extracellular matrix in astrocyte cultures."],"pmcid":["PMC12325430"],"funding_grant_id":["13/RC/2073-P2"],"pubmed_authors":["Zeugolis D","Kilcoyne M","McMahon SS","Nemati S"],"additional_accession":[]},"is_claimable":false,"name":"The effect of macromolecular crowders on deposition of extracellular matrix in astrocyte cultures.","description":"Macromolecular crowding (MMC) is a biophysical phenomenon that has proven effective in enhancing extracellular matrix (ECM) deposition in vitro. However, MMCs potential in neuroglial cell cultures remains underexplored. This study investigates the effects of three distinct MMC agents [carrageenan (CR), dextran sulphate (DxS) and FicollⓇ cocktail (FC)] on ECM deposition and cell behaviour of Neu7 and primary astrocytes. While the viability and metabolic activity of Neu7 astrocytes were unaffected by any of the crowding agents, primary astrocytes exhibited a significant decrease in viability and metabolic activity in the presence of CR and DxS. The addition of CR, DxS, and FC resulted in a significant increase in deposition of fibronectin, collagen IV, collagen I, GFAP and CS56 in Neu7 astro","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-07-15T09:03:59.715Z","creation":"2025-09-01T03:08:27.508Z"},"accession":"S-EPMC12325430","cross_references":{"pubmed":["40387922"],"doi":["10.1007/s00441-025-03980-4"]}}