{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["He J"],"funding":["the Science and Technology Innovation Leading Talent Project of the Central Plains of China"],"pagination":["508"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12326869"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["21(1)"],"pubmed_abstract":["<h4>Background</h4>African swine fever (ASF) is a highly lethal infectious disease caused by the African swine fever virus (ASFV). Due to the absence of effective vaccines and antiviral drugs, the slaughter of infected and exposed pigs is a current prevention and control measure. Early diagnosis is crucial for the management of ASFV, and the p15 protein plays a critical role in the maturation of ASFV particles.<h4>Results</h4>In this study, a prokaryotic expression system obtained highly pure soluble p15 protein, and four monoclonal antibodies (3 C, 4B, 5D, 8 F) were prepared. These antibodies demonstrated the specific recognition of exogenously expressed p15. Antigenic epitopes were initially mapped using six overlapping p15 truncated proteins. Results showed that the epitopes of 3 C, 5D,"],"journal":["BMC veterinary research"],"pubmed_title":["Preparation and identification of a novel monoclonal antibody against African swine fever virus p15 protein."],"pmcid":["PMC12326869"],"funding_grant_id":["244200510040"],"pubmed_authors":["Yao L","Liu Y","He J","Luo M","Hu X","Zhang M"],"additional_accession":[]},"is_claimable":false,"name":"Preparation and identification of a novel monoclonal antibody against African swine fever virus p15 protein.","description":"<h4>Background</h4>African swine fever (ASF) is a highly lethal infectious disease caused by the African swine fever virus (ASFV). Due to the absence of effective vaccines and antiviral drugs, the slaughter of infected and exposed pigs is a current prevention and control measure. Early diagnosis is crucial for the management of ASFV, and the p15 protein plays a critical role in the maturation of ASFV particles.<h4>Results</h4>In this study, a prokaryotic expression system obtained highly pure soluble p15 protein, and four monoclonal antibodies (3 C, 4B, 5D, 8 F) were prepared. These antibodies demonstrated the specific recognition of exogenously expressed p15. Antigenic epitopes were initially mapped using six overlapping p15 truncated proteins. Results showed that the epitopes of 3 C, 5D,","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-04-07T19:28:15.509Z","creation":"2026-04-07T17:09:20.654Z"},"accession":"S-EPMC12326869","cross_references":{"pubmed":["40770715"],"doi":["10.1186/s12917-025-04954-y"]}}