biostudies-literatureHe Jthe Science and Technology Innovation Leading Talent Project of the Central Plains of China508https://www.ebi.ac.uk/biostudies/studies/S-EPMC12326869biostudies-literatureUnknown21(1)<h4>Background</h4>African swine fever (ASF) is a highly lethal infectious disease caused by the African swine fever virus (ASFV). Due to the absence of effective vaccines and antiviral drugs, the slaughter of infected and exposed pigs is a current prevention and control measure. Early diagnosis is crucial for the management of ASFV, and the p15 protein plays a critical role in the maturation of ASFV particles.<h4>Results</h4>In this study, a prokaryotic expression system obtained highly pure soluble p15 protein, and four monoclonal antibodies (3 C, 4B, 5D, 8 F) were prepared. These antibodies demonstrated the specific recognition of exogenously expressed p15. Antigenic epitopes were initially mapped using six overlapping p15 truncated proteins. Results showed that the epitopes of 3 C, 5D,BMC veterinary researchPreparation and identification of a novel monoclonal antibody against African swine fever virus p15 protein.PMC12326869244200510040Yao LLiu YHe JLuo MHu XZhang MfalsePreparation and identification of a novel monoclonal antibody against African swine fever virus p15 protein.<h4>Background</h4>African swine fever (ASF) is a highly lethal infectious disease caused by the African swine fever virus (ASFV). Due to the absence of effective vaccines and antiviral drugs, the slaughter of infected and exposed pigs is a current prevention and control measure. Early diagnosis is crucial for the management of ASFV, and the p15 protein plays a critical role in the maturation of ASFV particles.<h4>Results</h4>In this study, a prokaryotic expression system obtained highly pure soluble p15 protein, and four monoclonal antibodies (3 C, 4B, 5D, 8 F) were prepared. These antibodies demonstrated the specific recognition of exogenously expressed p15. Antigenic epitopes were initially mapped using six overlapping p15 truncated proteins. Results showed that the epitopes of 3 C, 5D,2025-01-01T00:00:00Z2025 Aug2026-04-07T19:28:15.509Z2026-04-07T17:09:20.654ZS-EPMC123268694077071510.1186/s12917-025-04954-y