biostudies-literatureWang CShanghai Science and Technology Innovation Action PlanNatural Science Foundation of ShanghaiNatural Science Foundation of Shanghai MunicipalityNational Natural Science Foundation of ChinaShanghai Clinical Research Center for Gynecological Diseases747-773https://www.ebi.ac.uk/biostudies/studies/S-EPMC12328091biostudies-literatureUnknown45(7)<h4>Background</h4>Tumor-associated neutrophils (TANs) play a critical role in modulating immune responses and exhibit significant heterogeneity. Our previous study demonstrated that jagged canonical Notch ligand 2 (JAG2)⁺ TANs were associated with an immunosuppressive microenvironment in high-grade serous ovarian cancer (HGSOC), but the underlying mechanism remains unclear. This study aimed to elucidate the role of JAG2⁺ TANs in tumor immunosuppressive microenvironment in HGSOC.<h4>Methods</h4>HGSOC samples were collected, with 274 samples constituting two independent cohorts (training and validation cohorts) and an additional 30 samples utilized to establish patient-derived tumor organoids (PDTOs). We characterized the number and phenotype of JAG2⁺ TANs bCancer communications (London, England)Immunosuppressive JAG2&lt;sup&gt;+&lt;/sup&gt; tumor-associated neutrophils hamper PD-1 blockade response in ovarian cancer by mediating the differentiation of effector regulatory T cells.PMC12328091820728818220366523Y1190180023ZR140830022MC1940200822732058247327423Y11909500Zhang GLiu HCao KHuang YWang CYang MLu JHe MZhong YWang XZhang CWang YfalseImmunosuppressive JAG2&lt;sup&gt;+&lt;/sup&gt; tumor-associated neutrophils hamper PD-1 blockade response in ovarian cancer by mediating the differentiation of effector regulatory T cells.<h4>Background</h4>Tumor-associated neutrophils (TANs) play a critical role in modulating immune responses and exhibit significant heterogeneity. Our previous study demonstrated that jagged canonical Notch ligand 2 (JAG2)⁺ TANs were associated with an immunosuppressive microenvironment in high-grade serous ovarian cancer (HGSOC), but the underlying mechanism remains unclear. This study aimed to elucidate the role of JAG2⁺ TANs in tumor immunosuppressive microenvironment in HGSOC.<h4>Methods</h4>HGSOC samples were collected, with 274 samples constituting two independent cohorts (training and validation cohorts) and an additional 30 samples utilized to establish patient-derived tumor organoids (PDTOs). We characterized the number and phenotype of JAG2⁺ TANs b2025-01-01T00:00:00Z2025 Jul2026-07-15T12:45:21.293Z2026-07-04T03:16:31.229ZS-EPMC123280914012013910.1002/cac2.70021