biostudies-literatureAlptekin BValent Biosciences2328-2341https://www.ebi.ac.uk/biostudies/studies/S-EPMC12329162biostudies-literatureUnknown247(5)Glomalin, a substance produced by arbuscular mycorrhizal (AM) fungi, has well-documented benefits for plant and soil health, including water retention and soil aggregation. Glomalin quantification has been performed by enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody, MAb32B11, that has been described as targeting a heat shock protein 60 (RiHSP60). In this study, we re-examined the molecular nature of the antigen recognized by MAb32B11. MAb32B11 did not cross-react with the RiHSP60 polypeptide. Glomalin extracts of Rhizophagus irregularis showed strong and dose-dependent cross-reactivity with MAb32B11 even when protein levels were undetectable, raising doubts about the proteinaceous nature of the antigen. Protease treatments of glomalin extracts did not affect the ELISThe New phytologistFrom glomalin to glomalose: unraveling the molecular identity of the MAb32B11 antigen.PMC12329162MSN279975Hirsch HDaniels DAlptekin BKing LIrving TFloss DAne JMKleven BMcLimans CfalseFrom glomalin to glomalose: unraveling the molecular identity of the MAb32B11 antigen.Glomalin, a substance produced by arbuscular mycorrhizal (AM) fungi, has well-documented benefits for plant and soil health, including water retention and soil aggregation. Glomalin quantification has been performed by enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody, MAb32B11, that has been described as targeting a heat shock protein 60 (RiHSP60). In this study, we re-examined the molecular nature of the antigen recognized by MAb32B11. MAb32B11 did not cross-react with the RiHSP60 polypeptide. Glomalin extracts of Rhizophagus irregularis showed strong and dose-dependent cross-reactivity with MAb32B11 even when protein levels were undetectable, raising doubts about the proteinaceous nature of the antigen. Protease treatments of glomalin extracts did not affect the ELIS2025-01-01T00:00:00Z2025 Sep2026-04-12T19:25:00.841Z2026-04-07T13:17:34.813ZS-EPMC123291624047459810.1111/nph.70253