{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Dierlam C"],"funding":["National Cancer Institute","NCI NIH HHS","National Institute of General Medical Sciences","NIGMS NIH HHS","NIH HHS"],"pubmed_abstract":["Accurate cytokinesis is essential for maintaining genomic integrity. Although the GTPase dynamin has been well studied for its role in vesicular trafficking, its function during mitosis remains poorly understood. In this study, we uncover a novel role for the <i>C. elegans</i> dynamin homolog, DYN-1, in regulating mitotic spindle pole assembly and the spatiotemporal localization of the key mitotic kinase Polo-like kinase 1 (PLK-1). Our studies demonstrate that the depletion of DYN-1 leads to enlarged metaphase spindle poles and elevated levels of centrosome-associated PLK-1. Strikingly, PLK-1 fails to re-localize from centrosomes to the midbody during late mitosis in a subset of DYN-1-depleted embryos, correlating with abnormal PLK-1 localization at the midbody and defective midbody format"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2025.07.21.665896"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12330643"],"repository":["biostudies-literature"],"pubmed_title":["Dynamin regulates PLK-1 localization and spindle pole assembly during mitosis."],"pmcid":["PMC12330643"],"funding_grant_id":["5P20GM103636-09","R35 GM153737","P20 GM103636","7R15GM152965-02","R01 CA273095","1R35GM153737","1R01CA273095","P40 OD010440"],"pubmed_authors":["Newman RH","Held S","Anyanwu L","Iyer J","Dierlam C"],"additional_accession":[]},"is_claimable":false,"name":"Dynamin regulates PLK-1 localization and spindle pole assembly during mitosis.","description":"Accurate cytokinesis is essential for maintaining genomic integrity. Although the GTPase dynamin has been well studied for its role in vesicular trafficking, its function during mitosis remains poorly understood. In this study, we uncover a novel role for the <i>C. elegans</i> dynamin homolog, DYN-1, in regulating mitotic spindle pole assembly and the spatiotemporal localization of the key mitotic kinase Polo-like kinase 1 (PLK-1). Our studies demonstrate that the depletion of DYN-1 leads to enlarged metaphase spindle poles and elevated levels of centrosome-associated PLK-1. Strikingly, PLK-1 fails to re-localize from centrosomes to the midbody during late mitosis in a subset of DYN-1-depleted embryos, correlating with abnormal PLK-1 localization at the midbody and defective midbody format","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jul","modification":"2026-07-11T03:15:06.772Z","creation":"2026-07-11T03:08:22.265Z"},"accession":"S-EPMC12330643","cross_references":{"pubmed":["40777350"],"doi":["10.1101/2025.07.21.665896"]}}