biostudies-literatureUnknownChavez SRNICHD NIH HHSNIA NIH HHSNINDS NIH HHSNIGMS NIH HHSNIH HHSThe proper coordination of transcription factors, ATP dependent chromatin remodelers and histone modifications is essential for tissue specific gene expression, but how gene expression is regulated at these different levels is not well understood. In <i>C. elegans</i>, H3K4 methylation that is acquired in the germline is reprorgammed at fertilization by the H3K4me1/2 demethlyase SPR-5/LSD1/KDM1A and the H3K9 methyltransferase MET2/SETDB1/KMT2E. SPR-5/MET-2 maternal reprogramming is required to help establish the germline-soma distinction and prevent developmental delay by preventing inherited H3K4 methylation from inappropriately maintaining germline gene expression in somatic tissues. To determine if the DREAM transcriptional repressor complex and the MEC NuRD ATP dependent nucleosome rembioRxiv : the preprint server for biology2025.07.23.666413https://www.ebi.ac.uk/biostudies/studies/S-EPMC12330697biostudies-literatureThe Dream and MEC NuRD Complexes reinforce SPR-5/MET-2 maternal reprogramming to maintain the germline-soma distinction.PMC12330697T32 AG087922R15 GM148887T32 NS096050K12 GM000680P30 AG066511T34 GM140948T32 GM149422F32 HD097920P40 OD010440T32 GM008490F31 HD100145Schmeichel KLBlancher SDCarpenter BSChavez SRGosrani SPBirol OReeves MNMorgan-Benitez SLNguyen SKDozier JBrockett JSRodriguez JDKatz DJfalseThe Dream and MEC NuRD Complexes reinforce SPR-5/MET-2 maternal reprogramming to maintain the germline-soma distinction.The proper coordination of transcription factors, ATP dependent chromatin remodelers and histone modifications is essential for tissue specific gene expression, but how gene expression is regulated at these different levels is not well understood. In <i>C. elegans</i>, H3K4 methylation that is acquired in the germline is reprorgammed at fertilization by the H3K4me1/2 demethlyase SPR-5/LSD1/KDM1A and the H3K9 methyltransferase MET2/SETDB1/KMT2E. SPR-5/MET-2 maternal reprogramming is required to help establish the germline-soma distinction and prevent developmental delay by preventing inherited H3K4 methylation from inappropriately maintaining germline gene expression in somatic tissues. To determine if the DREAM transcriptional repressor complex and the MEC NuRD ATP dependent nucleosome rem2025-01-01T00:00:00Z2025 Jul2026-04-08T15:43:53.325Z2026-04-08T05:24:15.912ZS-EPMC123306974077726410.1101/2025.07.23.666413