{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Seol J"],"funding":["National Research Foundation of Korea funded by the Ministry of Science and ICT","Korea Health Technology R&D Project","Basic Science Research Institute Fund"],"pagination":["e2409933"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12332802"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["21(31)"],"pubmed_abstract":["A novel single-molecule immunoassay platform, termed DNA Hanger, is developed to address the limitations of conventional surface-based assays. By suspending biotinylated λ-phage DNA across microfabricated quartz barriers, this method enables high-specificity protein detection with minimal nonspecific binding. DNA Hanger significantly reduces background signals, achieving nonspecific binding rates as low as one protein per 236 µm of DNA. Quantification of mNeonGreen-tagged human poly(A)-binding protein C1 (mNG-PABP) and single-molecule fluorescence-linked immunosorbent assay (FLISA) of human tumor necrosis factor α (TNF-α) demonstrates the assay's specificity and sensitivity at the single-molecule level, with a detection limit of 0.90 pM in buffer, 38-fold lower than that of conventional FLISA, and 20.6 pM in 70% fetal bovine serum, an 8-fold improvement. DNA Hanger also enables the detection and quantification of endogenous TNF-α in human serum, highlighting its clinical potential. The DNA Hanger assay eliminates the need for surface blocking and simplifies workflow, resulting in completing the immunoassay process within 1 hour. DNA Hanger offers broad applicability for biomolecular interaction studies and clinical diagnostics."],"journal":["Small (Weinheim an der Bergstrasse, Germany)"],"pubmed_title":["DNA Hanger: Surface-Minimized Single-Molecule Immunoassay Platform."],"pmcid":["PMC12332802"],"funding_grant_id":["RX2023-00266133","RS-2023-00218927","RS-2021-NR060139","2021R1A2C1095046"],"pubmed_authors":["Lee JB","Kim B","Jeong C","Yu ES","Seol J"],"additional_accession":[]},"is_claimable":false,"name":"DNA Hanger: Surface-Minimized Single-Molecule Immunoassay Platform.","description":"A novel single-molecule immunoassay platform, termed DNA Hanger, is developed to address the limitations of conventional surface-based assays. By suspending biotinylated λ-phage DNA across microfabricated quartz barriers, this method enables high-specificity protein detection with minimal nonspecific binding. DNA Hanger significantly reduces background signals, achieving nonspecific binding rates as low as one protein per 236 µm of DNA. Quantification of mNeonGreen-tagged human poly(A)-binding protein C1 (mNG-PABP) and single-molecule fluorescence-linked immunosorbent assay (FLISA) of human tumor necrosis factor α (TNF-α) demonstrates the assay's specificity and sensitivity at the single-molecule level, with a detection limit of 0.90 pM in buffer, 38-fold lower than that of conventional FLISA, and 20.6 pM in 70% fetal bovine serum, an 8-fold improvement. DNA Hanger also enables the detection and quantification of endogenous TNF-α in human serum, highlighting its clinical potential. The DNA Hanger assay eliminates the need for surface blocking and simplifies workflow, resulting in completing the immunoassay process within 1 hour. DNA Hanger offers broad applicability for biomolecular interaction studies and clinical diagnostics.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-05-01T08:37:11.798Z","creation":"2026-04-19T03:07:45.334Z"},"accession":"S-EPMC12332802","cross_references":{"pubmed":["40484729"],"doi":["10.1002/smll.202409933"]}}