biostudies-literatureKong FNatural Science Foundation of Zhejiang ProvinceNational Natural Science Foundation of ChinaScience and Technology Department of Zhejiang ProvinceNational Key Research and Development Program of China27159-27172https://www.ebi.ac.uk/biostudies/studies/S-EPMC12333425biostudies-literatureUnknown19(30)Plastic pollution is a growing global issue, with nanoplastics (NPs) posing a greater threat than microplastics. Micro/nanoplastics have been detected in various human tissues and bodily fluids, but their impacts on human fertility remain unclear. We used pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) with liquid extraction to detect NPs in the follicular fluid (FF) and seminal plasma (SP) of 51 couples undergoing in vitro fertilization (IVF) to investigate the impact of NPs on fertility. In our study, polyethylene (PE) and polyvinyl chloride (PVC) were the most frequently detected NPs. In FF, the average PE and PVC concentrations were 1.21 μg/g and 1.85 μg/g, respectively, whereas in SP, they were 3.02 μg/g and 2.67 μg/g, respectively. For NPs in FF, no significant association ACS nanoPolyethylene and Polyvinyl Chloride Nanoplastics in Human Follicular Fluid and Seminal Plasma: Impact on Fertilization and Sperm Quality.PMC123334258220175922306160LMS25H040001824019502023C030342025C021232022YFC2702300Jiang LZhang YZhao PKong FTong XGui YZhang SFang LLan HfalsePolyethylene and Polyvinyl Chloride Nanoplastics in Human Follicular Fluid and Seminal Plasma: Impact on Fertilization and Sperm Quality.Plastic pollution is a growing global issue, with nanoplastics (NPs) posing a greater threat than microplastics. Micro/nanoplastics have been detected in various human tissues and bodily fluids, but their impacts on human fertility remain unclear. We used pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) with liquid extraction to detect NPs in the follicular fluid (FF) and seminal plasma (SP) of 51 couples undergoing in vitro fertilization (IVF) to investigate the impact of NPs on fertility. In our study, polyethylene (PE) and polyvinyl chloride (PVC) were the most frequently detected NPs. In FF, the average PE and PVC concentrations were 1.21 μg/g and 1.85 μg/g, respectively, whereas in SP, they were 3.02 μg/g and 2.67 μg/g, respectively. For NPs in FF, no significant association 2025-01-01T00:00:00Z2025 Aug2026-05-01T08:40:26.446Z2026-04-19T03:08:10.451ZS-EPMC123334254068976310.1021/acsnano.5c00918