biostudies-literatureUnknownAlcicek SMildred-Scheel Career Center of the German Cancer AidDiscovery & Development Project Grant of the LOEWE Frankfurt Cancer Institute<h4>Background</h4>Glutamate and glutamine are critical metabolites in gliomas, each serving distinct roles in tumor biology. Separate quantification of these metabolites using in vivo MR spectroscopy (MRS) at clinical field strengths (≤ 3T) is hindered by their molecular similarity, resulting in overlapping, hence indistinguishable, spectral peaks.<h4>Purpose</h4>To develop an MRS imaging (MRSI) protocol to map glutamate and glutamine separately at 3T within clinically feasible time, using J-modulation to enhance spectral differentiation, demonstrate its reliability/reproducibility, and quantify the metabolites in glioma subregions.<h4>Study type</h4>Prospective.<h4>Population</h4>Phantoms, 5 healthy subjects, and 30 patients with suspected glioma. IDH wild-type glioblastoma cases were evJournal of magnetic resonance imaging : JMRIhttps://www.ebi.ac.uk/biostudies/studies/S-EPMC12335340biostudies-literatureOptimized Long-TE &lt;sup&gt;1&lt;/sup&gt;H sLASER MR Spectroscopic Imaging at 3T for Separate Quantification of Glutamate and Glutamine in Glioma.PMC12335340Ronellenfitsch MWWeber KJThomas DCManzhurtsev APilatus UWenger KJSteinbach JPHattingen EForster MTAlcicek SPrinz VfalseOptimized Long-TE &lt;sup&gt;1&lt;/sup&gt;H sLASER MR Spectroscopic Imaging at 3T for Separate Quantification of Glutamate and Glutamine in Glioma.<h4>Background</h4>Glutamate and glutamine are critical metabolites in gliomas, each serving distinct roles in tumor biology. Separate quantification of these metabolites using in vivo MR spectroscopy (MRS) at clinical field strengths (≤ 3T) is hindered by their molecular similarity, resulting in overlapping, hence indistinguishable, spectral peaks.<h4>Purpose</h4>To develop an MRS imaging (MRSI) protocol to map glutamate and glutamine separately at 3T within clinically feasible time, using J-modulation to enhance spectral differentiation, demonstrate its reliability/reproducibility, and quantify the metabolites in glioma subregions.<h4>Study type</h4>Prospective.<h4>Population</h4>Phantoms, 5 healthy subjects, and 30 patients with suspected glioma. IDH wild-type glioblastoma cases were ev2025-01-01T00:00:00Z2025 Apr2026-05-01T08:47:09.933Z2026-04-07T16:45:27.427ZS-EPMC123353404019780810.1002/jmri.29787