{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["24(8)"],"submitter":["Scalia E"],"funding":["European Commission"],"pubmed_abstract":["Alzheimer's disease (AD) is a multifactorial neurodegenerative disorder that presents with heterogeneous clinical and pathological features, necessitating improved biomarkers for accurate diagnosis and patient stratification. In this study, we applied a data-independent acquisition-based proteomics workflow to cerebrospinal fluid (CSF) samples from 138 individuals, including AD patients with high (Aβ+/tau+) or normal (Aβ+/tau-) CSF tau levels, and non-AD controls. Analysis using an Astral mass spectrometer enabled unprecedented proteome depth, identifying 2661 proteins with high data completeness. Comparative proteomic profiling revealed distinct protein signatures for Aβ+/tau+ and Aβ+/tau- subtypes. These findings were validated using an independent internal cohort and further corroborate"],"journal":["Molecular & cellular proteomics : MCP"],"pagination":["101025"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12335992"],"repository":["biostudies-literature"],"pubmed_title":["Proteome Profiling of Cerebrospinal Fluid and Machine Learning Reveal Protein Classifiers of Two Forms of Alzheimer's Disease Characterized by Increased or Not Altered Levels of Tau."],"pmcid":["PMC12335992"],"pubmed_authors":["Piccoli T","Lo Pinto M","Iemmolo M","Scilabra SD","Ghersi G","Scalia E","Lo Re V","Castelbuono S","Bivona G","Calligaris M"],"additional_accession":[]},"is_claimable":false,"name":"Proteome Profiling of Cerebrospinal Fluid and Machine Learning Reveal Protein Classifiers of Two Forms of Alzheimer's Disease Characterized by Increased or Not Altered Levels of Tau.","description":"Alzheimer's disease (AD) is a multifactorial neurodegenerative disorder that presents with heterogeneous clinical and pathological features, necessitating improved biomarkers for accurate diagnosis and patient stratification. In this study, we applied a data-independent acquisition-based proteomics workflow to cerebrospinal fluid (CSF) samples from 138 individuals, including AD patients with high (Aβ+/tau+) or normal (Aβ+/tau-) CSF tau levels, and non-AD controls. Analysis using an Astral mass spectrometer enabled unprecedented proteome depth, identifying 2661 proteins with high data completeness. Comparative proteomic profiling revealed distinct protein signatures for Aβ+/tau+ and Aβ+/tau- subtypes. These findings were validated using an independent internal cohort and further corroborate","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-07-06T03:14:35.998Z","creation":"2026-07-06T03:08:38.668Z"},"accession":"S-EPMC12335992","cross_references":{"pubmed":["40602528"],"doi":["10.1016/j.mcpro.2025.101025"]}}