biostudies-literatureYan Fthe Natural Science Foundation of Shandong ProvinceResearch Fund for Lin He's Academician Workstation of New Medicine and Clinical Translation at Jining Medical UniversityProject of Medical and Health Technology Development Program in Shandong ProvinceNational Natural Science Foundation of ChinaShandong Training Program of Innovation and Entrepreneurship for UndergraduatesProject of Shandong Province Higher Educational Youth Innovation Science and Technology ProgramTai Shan Young Scholar Foundation of Shandong ProvinceResearch Fund for Lin He’s Academician Workstation of New Medicine and Clinical Translation at Jining Medical University2274-2291https://www.ebi.ac.uk/biostudies/studies/S-EPMC12336090biostudies-literatureUnknown48(4)Sappanone A (SAP), a high-isoflavone compound derived from the traditional Chinese medicine Sumu, exhibits various pharmacological activities, including anti-inflammatory and anti-oxidant effects. However, its protective effects on the liver have rarely been reported. The aim of this study was to investigate the effects of SAP on immune-mediated liver injury induced by concanavalin A (Con A) in mice and to explore the underlying molecular mechanisms. Mice were administered SAP intraperitoneally (50 mg/kg body weight). Three hours later, Con A (18 mg/kg) was injected via the tail vein to induce liver damage. Livers and blood were collected 12 h after Con A challenge. Liver cell apoptosis, oxidative stress, and M1 macrophage activation in vivo were investigated. Bone marrow-derived macrophagInflammationSappanone A Ameliorates Concanavalin A-induced Immune-Mediated Liver Injury by Regulating M1 Macrophage Polarization.PMC12336090S20241044320182171810202202070388JYHL2022MS21ZR2021MH287ZR2023QH332tsqn2022112342022020506782021KJ074Guo ZLi CZhang JWang CZhang HSun XZhong ZWang LLi WLiu ZDong GGao MChen SYan FXiong HfalseSappanone A Ameliorates Concanavalin A-induced Immune-Mediated Liver Injury by Regulating M1 Macrophage Polarization.Sappanone A (SAP), a high-isoflavone compound derived from the traditional Chinese medicine Sumu, exhibits various pharmacological activities, including anti-inflammatory and anti-oxidant effects. However, its protective effects on the liver have rarely been reported. The aim of this study was to investigate the effects of SAP on immune-mediated liver injury induced by concanavalin A (Con A) in mice and to explore the underlying molecular mechanisms. Mice were administered SAP intraperitoneally (50 mg/kg body weight). Three hours later, Con A (18 mg/kg) was injected via the tail vein to induce liver damage. Livers and blood were collected 12 h after Con A challenge. Liver cell apoptosis, oxidative stress, and M1 macrophage activation in vivo were investigated. Bone marrow-derived macrophag2025-01-01T00:00:00Z2025 Aug2026-04-30T14:37:20.065Z2026-04-07T16:03:40.376ZS-EPMC123360903958963410.1007/s10753-024-02189-x