biostudies-literatureShi YJapan Agency for Medical Research and DevelopmentJapan Science and Technology AgencyJapan Society for the Promotion of Science8580-8585https://www.ebi.ac.uk/biostudies/studies/S-EPMC12340962biostudies-literatureUnknown27(31)Genome mining of polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) in fungal endophyte <i>Neopestalotiopsis olumideae</i> strain BRIP 39872 identified seven new natural products (olumilides A-D, D1, D1', D2) with pyrano[2,3-<i>c</i>]pyrrole scaffolds and their biosynthetic precursor preolumilide. Unlike known congeners, such as curvupallides, olumilides possess alkyl side chains with branched methyl groups and threonine-derived core scaffolds. Absolute configurations were determined by microED and ECD simulations, providing valuable information for further research focusing on the stereochemistry of PKS-NRPS reactions.Organic lettersGenome Mining-Based Discovery of Pyrano[2,3-&lt;i&gt;c&lt;/i&gt;]pyrrole Type Natural Products Possessing Alkyl Side Chain with Branched Methyl Groups.PMC12340962JP24ama121040JPMJAN23D4JP22H02775JP24ama121038JPMJFR205WJP23gm1610007Fukano HAsai TSuzuki MKodama ENMatsuda RHoshino YShimotai YAoyama YDavis RAOzaki TTaniguchi TFuse TShi YYasuda JNumata KYoshikawa RHayashi HHamamoto HMorishita YHirabayashi AHosotani KSato HSugawara AfalseGenome Mining-Based Discovery of Pyrano[2,3-&lt;i&gt;c&lt;/i&gt;]pyrrole Type Natural Products Possessing Alkyl Side Chain with Branched Methyl Groups.Genome mining of polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) in fungal endophyte <i>Neopestalotiopsis olumideae</i> strain BRIP 39872 identified seven new natural products (olumilides A-D, D1, D1', D2) with pyrano[2,3-<i>c</i>]pyrrole scaffolds and their biosynthetic precursor preolumilide. Unlike known congeners, such as curvupallides, olumilides possess alkyl side chains with branched methyl groups and threonine-derived core scaffolds. Absolute configurations were determined by microED and ECD simulations, providing valuable information for further research focusing on the stereochemistry of PKS-NRPS reactions.2025-01-01T00:00:00Z2025 Aug2026-04-16T15:09:22.422Z2026-04-07T14:12:58.07ZS-EPMC123409624077669810.1021/acs.orglett.5c02504