{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["15(8)"],"submitter":["Nalley MJ"],"funding":["R01 AI000272"],"pubmed_abstract":["We recently described CRISPR/Cas9-based short homology-dependent genome engineering in the human fungal pathogen Cryptococcus neoformans, a haploid budding yeast that is the most common cause of fungal meningitis and an emerging model organism. This was achieved by electroporation of strains stably expressing a codon-optimized Cas9 with 2 separate DNA molecules, one encoding a selectable marker flanked by short homology arms and a second encoding a sgRNA under the control of the U6 snRNA promoter. However, the efficiency of desired homology-dependent repair relative to undesired non-homologous end-joining (NHEJ) events can be low and variable. Here, we describe methods and strains enabling extremely efficient (∼99%) homology-dependent genome editing in C. neoformans. This high-efficiency m"],"journal":["G3 (Bethesda, Md.)"],"pagination":["jkaf118"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12341884"],"repository":["biostudies-literature"],"pubmed_title":["Near 100% efficient homology-dependent genome engineering in the human fungal pathogen Cryptococcus neoformans."],"pmcid":["PMC12341884"],"pubmed_authors":["Banerjee S","Nalley MJ","Huang MY","Madhani HD"],"additional_accession":[]},"is_claimable":false,"name":"Near 100% efficient homology-dependent genome engineering in the human fungal pathogen Cryptococcus neoformans.","description":"We recently described CRISPR/Cas9-based short homology-dependent genome engineering in the human fungal pathogen Cryptococcus neoformans, a haploid budding yeast that is the most common cause of fungal meningitis and an emerging model organism. This was achieved by electroporation of strains stably expressing a codon-optimized Cas9 with 2 separate DNA molecules, one encoding a selectable marker flanked by short homology arms and a second encoding a sgRNA under the control of the U6 snRNA promoter. However, the efficiency of desired homology-dependent repair relative to undesired non-homologous end-joining (NHEJ) events can be low and variable. Here, we describe methods and strains enabling extremely efficient (∼99%) homology-dependent genome editing in C. neoformans. This high-efficiency m","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-04-07T18:29:47.159Z","creation":"2026-04-07T16:35:41.391Z"},"accession":"S-EPMC12341884","cross_references":{"pubmed":["40460280"],"doi":["10.1093/g3journal/jkaf118"]}}