biostudies-literaturePatti SBiotechnology and Biological Sciences Research Council180https://www.ebi.ac.uk/biostudies/studies/S-EPMC12343752biostudies-literatureUnknown109(1)A (S)-selective amine transaminase from a Streptomyces strain, Sbv333-ATA, is a biocatalyst showing both high thermostability with a melting temperature of 85 °C and broad substrate specificity for the amino acceptor. This enzyme was further characterized both biochemically and structurally. The Sbv333-ATA is stable in the presence of up to 20% (v/v) of the water-miscible cosolvents methanol, ethanol, acetonitrile, and dimethyl sulfoxide, and in biphasic systems with petroleum ether, toluene, and ethyl acetate as an organic phase. The enzyme showed also a good activity toward different amino donors, such as (S)-methylbenzylamine and 2-phenylethylamine, aliphatic mono- and di-amines, like propylamine and cadaverine, and selected amino acids. However, more sterically hindered aromatic aminesApplied microbiology and biotechnologyFunctional and structural insights into a thermostable (S)-selective amine transaminase and its improved substrate scope by protein engineering.PMC12343752BB/L002035/1Isupov MNDe Rose SAMagrini Alunno IRiva SPatti SFerrandi EELittlechild JAMonti DfalseFunctional and structural insights into a thermostable (S)-selective amine transaminase and its improved substrate scope by protein engineering.A (S)-selective amine transaminase from a Streptomyces strain, Sbv333-ATA, is a biocatalyst showing both high thermostability with a melting temperature of 85 °C and broad substrate specificity for the amino acceptor. This enzyme was further characterized both biochemically and structurally. The Sbv333-ATA is stable in the presence of up to 20% (v/v) of the water-miscible cosolvents methanol, ethanol, acetonitrile, and dimethyl sulfoxide, and in biphasic systems with petroleum ether, toluene, and ethyl acetate as an organic phase. The enzyme showed also a good activity toward different amino donors, such as (S)-methylbenzylamine and 2-phenylethylamine, aliphatic mono- and di-amines, like propylamine and cadaverine, and selected amino acids. However, more sterically hindered aromatic amines2025-01-01T00:00:00Z2025 Aug2026-06-08T06:51:36.944Z2026-06-08T03:14:37.088ZS-EPMC123437524079698110.1007/s00253-025-13536-9