biostudies-literatureMoon HMinistry of EducationUnited States Department of Agriculture and HatchMinistry of Science and ICT, South KoreaNational Research Foundation of KoreaNational Institutes of HealthNIH HHSNIGMS NIH HHSThe National Institute of Food and Agriculture, the National Institute of Agricultural SciencesNational Science Foundatione0118125https://www.ebi.ac.uk/biostudies/studies/S-EPMC12345274biostudies-literatureUnknown16(8)The evolutionarily conserved GATA-type transcription factor (TF) NsdD regulates sexual and asexual development as well as secondary metabolism in various <i>Aspergillus</i> species. Despite its well-known multifunctionality, the mechanisms by which NsdD coordinates such diverse biological processes remain unclear. To address this gap, we have conducted network-based multiomics analyses in two distantly related species, <i>Aspergillus nidulans</i> and <i>Aspergillus flavus</i>. Transcriptomic profiling reveals that NsdD regulates gene expression in a cell type- and species-specific manner. The potential evolutionary conservation of NsdD was tested by a cross-complementation experiment in which the <i>A. nidulans nsdD</i> gene was introduced into the <i>A. flavus</i> Δ<i>nsdD</i> mutant. ThimBioSpecies-specific gene regulatory network rewiring mediated by the GATA-type regulator NsdD in &lt;i&gt;Aspergillus&lt;/i&gt;.PMC12345274R01 GM1127392022-DD-RD-0574-02RS-2024-003396597000326PJ015298R01 2R01GM112739-05A117157102017R1D1A3B06035312Amador-Noguez DLee M-KPark SCKeller NPShin JRivera Vazquez JCMoon HPark H-SYu J-HHan K-HfalseSpecies-specific gene regulatory network rewiring mediated by the GATA-type regulator NsdD in &lt;i&gt;Aspergillus&lt;/i&gt;.The evolutionarily conserved GATA-type transcription factor (TF) NsdD regulates sexual and asexual development as well as secondary metabolism in various <i>Aspergillus</i> species. Despite its well-known multifunctionality, the mechanisms by which NsdD coordinates such diverse biological processes remain unclear. To address this gap, we have conducted network-based multiomics analyses in two distantly related species, <i>Aspergillus nidulans</i> and <i>Aspergillus flavus</i>. Transcriptomic profiling reveals that NsdD regulates gene expression in a cell type- and species-specific manner. The potential evolutionary conservation of NsdD was tested by a cross-complementation experiment in which the <i>A. nidulans nsdD</i> gene was introduced into the <i>A. flavus</i> Δ<i>nsdD</i> mutant. Thi2025-01-01T00:00:00Z2025 Aug2026-04-18T11:21:11.219Z2026-04-07T14:35:35.338ZS-EPMC123452744060780810.1128/mbio.01181-25