{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kunieda S"],"funding":["Ministry of Culture, Sports, Science and Technology"],"pagination":["1154"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12346565"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(15)"],"pubmed_abstract":["Human adipose-derived stem cells (hASCs) are widely used in regenerative medicine due to their accessibility and high proliferative capacity. Platelet lysate (PL) has recently emerged as a promising alternative to fetal bovine serum (FBS), offering superior cell expansion potential; however, the molecular basis for its efficacy remains insufficiently elucidated. In this study, we performed RNA sequencing to compare hASCs cultured with PL or FBS, revealing a significant upregulation of genes related to stress response and cell proliferation under PL conditions. These findings were validated by RT-qPCR and supported by functional assays demonstrating enhanced cellular resilience to oxidative and genotoxic stress, reduced doxorubicin-induced senescence, and improved antiapoptotic properties. "],"journal":["Cells"],"pubmed_title":["Molecular Insights into the Superiority of Platelet Lysate over FBS for hASC Expansion and Wound Healing."],"pmcid":["PMC12346565"],"funding_grant_id":["22K09899"],"pubmed_authors":["Li H","Kakudo N","Taketani S","Mitsui T","Higasa K","Fukui M","Kuro A","Ueda T","Kunieda S","Sun Z"],"additional_accession":[]},"is_claimable":false,"name":"Molecular Insights into the Superiority of Platelet Lysate over FBS for hASC Expansion and Wound Healing.","description":"Human adipose-derived stem cells (hASCs) are widely used in regenerative medicine due to their accessibility and high proliferative capacity. Platelet lysate (PL) has recently emerged as a promising alternative to fetal bovine serum (FBS), offering superior cell expansion potential; however, the molecular basis for its efficacy remains insufficiently elucidated. In this study, we performed RNA sequencing to compare hASCs cultured with PL or FBS, revealing a significant upregulation of genes related to stress response and cell proliferation under PL conditions. These findings were validated by RT-qPCR and supported by functional assays demonstrating enhanced cellular resilience to oxidative and genotoxic stress, reduced doxorubicin-induced senescence, and improved antiapoptotic properties. ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jul","modification":"2026-07-04T03:21:21.041Z","creation":"2026-07-04T03:14:40.741Z"},"accession":"S-EPMC12346565","cross_references":{"pubmed":["40801587"],"doi":["10.3390/cells14151154"]}}