biostudies-literatureKunieda SMinistry of Culture, Sports, Science and Technology1154https://www.ebi.ac.uk/biostudies/studies/S-EPMC12346565biostudies-literatureUnknown14(15)Human adipose-derived stem cells (hASCs) are widely used in regenerative medicine due to their accessibility and high proliferative capacity. Platelet lysate (PL) has recently emerged as a promising alternative to fetal bovine serum (FBS), offering superior cell expansion potential; however, the molecular basis for its efficacy remains insufficiently elucidated. In this study, we performed RNA sequencing to compare hASCs cultured with PL or FBS, revealing a significant upregulation of genes related to stress response and cell proliferation under PL conditions. These findings were validated by RT-qPCR and supported by functional assays demonstrating enhanced cellular resilience to oxidative and genotoxic stress, reduced doxorubicin-induced senescence, and improved antiapoptotic properties. CellsMolecular Insights into the Superiority of Platelet Lysate over FBS for hASC Expansion and Wound Healing.PMC1234656522K09899Li HKakudo NTaketani SMitsui THigasa KFukui MKuro AUeda TKunieda SSun ZfalseMolecular Insights into the Superiority of Platelet Lysate over FBS for hASC Expansion and Wound Healing.Human adipose-derived stem cells (hASCs) are widely used in regenerative medicine due to their accessibility and high proliferative capacity. Platelet lysate (PL) has recently emerged as a promising alternative to fetal bovine serum (FBS), offering superior cell expansion potential; however, the molecular basis for its efficacy remains insufficiently elucidated. In this study, we performed RNA sequencing to compare hASCs cultured with PL or FBS, revealing a significant upregulation of genes related to stress response and cell proliferation under PL conditions. These findings were validated by RT-qPCR and supported by functional assays demonstrating enhanced cellular resilience to oxidative and genotoxic stress, reduced doxorubicin-induced senescence, and improved antiapoptotic properties. 2025-01-01T00:00:00Z2025 Jul2026-07-04T03:21:21.041Z2026-07-04T03:14:40.741ZS-EPMC123465654080158710.3390/cells14151154