{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["108(3)"],"submitter":["Indrawattana N"],"pubmed_abstract":["ObjectiveThis study aimed to characterize the genetic diversity, structural variation, and functional role of the cycle inhibiting factor (Cif) in <i>Burkholderia pseudomallei</i>, with a particular focus on its involvement in neuronal infections.MethodsWe analyzed the <i>cif</i> gene (<i>bpss1385</i>) from 1294 clinical isolates of <i>B. pseudomallei</i> using phylogenetic analysis and structural modeling to identify Cif variant types. Functional characterization of selected variants was performed using plaque formation assays in SH-SY5Y neuroblastoma cells. Additionally, proteomic profiling was conducted to assess differential host protein expression in SH-SY5Y cells infected with the <i>B. pseudomallei</i> strain K96243 versus a <i>cif</i>-deleted mutant.ResultsThe <i>cif</i> gene was p"],"journal":["Science progress"],"pagination":["368504251369011"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12351145"],"repository":["biostudies-literature"],"pubmed_title":["Deciphering the diversity, structure, and function of cycle-inhibiting factor in &lt;i&gt;Burkholderia pseudomallei&lt;/i&gt; neuroinfection."],"pmcid":["PMC12351145"],"pubmed_authors":["Pumirat P","Chantratita N","Reamtong O","Santajit S","Rungruengkitkun A","Kong-Ngoen T","Indrawattana N","Tunyong W","Seng R","Sricharunrat T","Thavorasak T"],"additional_accession":[]},"is_claimable":false,"name":"Deciphering the diversity, structure, and function of cycle-inhibiting factor in &lt;i&gt;Burkholderia pseudomallei&lt;/i&gt; neuroinfection.","description":"ObjectiveThis study aimed to characterize the genetic diversity, structural variation, and functional role of the cycle inhibiting factor (Cif) in <i>Burkholderia pseudomallei</i>, with a particular focus on its involvement in neuronal infections.MethodsWe analyzed the <i>cif</i> gene (<i>bpss1385</i>) from 1294 clinical isolates of <i>B. pseudomallei</i> using phylogenetic analysis and structural modeling to identify Cif variant types. Functional characterization of selected variants was performed using plaque formation assays in SH-SY5Y neuroblastoma cells. Additionally, proteomic profiling was conducted to assess differential host protein expression in SH-SY5Y cells infected with the <i>B. pseudomallei</i> strain K96243 versus a <i>cif</i>-deleted mutant.ResultsThe <i>cif</i> gene was p","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jul-Sep","modification":"2026-07-15T09:03:34.597Z","creation":"2026-07-02T03:08:27.704Z"},"accession":"S-EPMC12351145","cross_references":{"pubmed":["40801845"],"doi":["10.1177/00368504251369011"]}}