biostudies-literatureUnknown108(3)Indrawattana NObjectiveThis study aimed to characterize the genetic diversity, structural variation, and functional role of the cycle inhibiting factor (Cif) in <i>Burkholderia pseudomallei</i>, with a particular focus on its involvement in neuronal infections.MethodsWe analyzed the <i>cif</i> gene (<i>bpss1385</i>) from 1294 clinical isolates of <i>B. pseudomallei</i> using phylogenetic analysis and structural modeling to identify Cif variant types. Functional characterization of selected variants was performed using plaque formation assays in SH-SY5Y neuroblastoma cells. Additionally, proteomic profiling was conducted to assess differential host protein expression in SH-SY5Y cells infected with the <i>B. pseudomallei</i> strain K96243 versus a <i>cif</i>-deleted mutant.ResultsThe <i>cif</i> gene was pScience progress368504251369011https://www.ebi.ac.uk/biostudies/studies/S-EPMC12351145biostudies-literatureDeciphering the diversity, structure, and function of cycle-inhibiting factor in &lt;i&gt;Burkholderia pseudomallei&lt;/i&gt; neuroinfection.PMC12351145Pumirat PChantratita NReamtong OSantajit SRungruengkitkun AKong-Ngoen TIndrawattana NTunyong WSeng RSricharunrat TThavorasak TfalseDeciphering the diversity, structure, and function of cycle-inhibiting factor in &lt;i&gt;Burkholderia pseudomallei&lt;/i&gt; neuroinfection.ObjectiveThis study aimed to characterize the genetic diversity, structural variation, and functional role of the cycle inhibiting factor (Cif) in <i>Burkholderia pseudomallei</i>, with a particular focus on its involvement in neuronal infections.MethodsWe analyzed the <i>cif</i> gene (<i>bpss1385</i>) from 1294 clinical isolates of <i>B. pseudomallei</i> using phylogenetic analysis and structural modeling to identify Cif variant types. Functional characterization of selected variants was performed using plaque formation assays in SH-SY5Y neuroblastoma cells. Additionally, proteomic profiling was conducted to assess differential host protein expression in SH-SY5Y cells infected with the <i>B. pseudomallei</i> strain K96243 versus a <i>cif</i>-deleted mutant.ResultsThe <i>cif</i> gene was p2025-01-01T00:00:00Z2025 Jul-Sep2026-07-15T09:03:34.597Z2026-07-02T03:08:27.704ZS-EPMC123511454080184510.1177/00368504251369011