{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Uchida K"],"funding":["Japan Society for the Promotion of Science"],"pubmed_abstract":["Protein lipidation, particularly palmitoylation (attachment of a 16-carbon fatty acid), regulates cellular behaviors by controlling protein function at lipid membranes. In this study, we prepared a series of lipidated green fluorescent proteins (\"EGFP-lipids\") with various alkyl chain lengths (C8 to C22). Using model lipid membranes and Jurkat (human T lymphocyte) cells, we evaluated how lipidation affects the membrane interactions and vesicular transport from the membrane of these protein-lipid constructs. Our findings demonstrate that elongation of the alkyl chain profoundly affects both lateral membrane diffusion and vesicular transport of the EGFP-lipids. Only artificially lipidated proteins that mimic <i>in vivo</i> lipidation exhibited cellular dynamics in response to external signal"],"journal":["RSC chemical biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12351397"],"repository":["biostudies-literature"],"pubmed_title":["Exploring the significance of palmitoylation using an artificial protein lipidation system."],"pmcid":["PMC12351397"],"funding_grant_id":["JP23KJ1739","JP19H00841","JP23H00247"],"pubmed_authors":["Shimokawa N","Shiomoto S","Goto M","Uchida K","Wakabayashi R","Kamiya N","Toyofuku K","Ogushi N","Tanaka M","Takagi M"],"additional_accession":[]},"is_claimable":false,"name":"Exploring the significance of palmitoylation using an artificial protein lipidation system.","description":"Protein lipidation, particularly palmitoylation (attachment of a 16-carbon fatty acid), regulates cellular behaviors by controlling protein function at lipid membranes. In this study, we prepared a series of lipidated green fluorescent proteins (\"EGFP-lipids\") with various alkyl chain lengths (C8 to C22). Using model lipid membranes and Jurkat (human T lymphocyte) cells, we evaluated how lipidation affects the membrane interactions and vesicular transport from the membrane of these protein-lipid constructs. Our findings demonstrate that elongation of the alkyl chain profoundly affects both lateral membrane diffusion and vesicular transport of the EGFP-lipids. Only artificially lipidated proteins that mimic <i>in vivo</i> lipidation exhibited cellular dynamics in response to external signal","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-05-28T04:14:27.089Z","creation":"2026-04-08T02:15:43.02Z"},"accession":"S-EPMC12351397","cross_references":{"pubmed":["40821863"],"doi":["10.1039/d5cb00143a"]}}