{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sripada A"],"funding":["NIAID NIH HHS"],"pagination":["e187907"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12352889"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["135(16)"],"pubmed_abstract":["The mechanisms of neutrophilic and mixed neutrophilic-eosinophilic asthma are poorly understood. We found that extracellular DNA and nucleosomes (Nucs) were elevated in the airways of patients with neutrophilic-eosinophilic asthma and correlated with bronchoalveolar lavage neutrophils. Bronchial tissue from neutrophilic-eosinophilic asthma had more DNA sensor-positive cells. Intranasally administered DNA did not induce airway hyperreactivity (AHR) or any pathology but induced AHR and neutrophilic-eosinophilic inflammation when coadministered with the allergen Alternaria (Alt). Nuc alone induced antiinflammatory/defensive genes, whereas the Nuc-Alt combination increased levels of TNF-α and innate cytokines. The Alt-Nuc phenotype was abolished in Cgas-/-, ALR-/-, Sting-/-, LysMCre:Stingfl/fl"],"journal":["The Journal of clinical investigation"],"pubmed_title":["Allergens abrogate antiinflammatory DNA effects and unmask macrophage-driven neutrophilic asthma via ILC2/STING/TNF-α signaling."],"pmcid":["PMC12352889"],"funding_grant_id":["R01 AI157138","R01 AI165922","R01 AI137970"],"pubmed_authors":["Alam R","Pathria M","Martin RJ","Kwiat C","Getahun A","Verma M","Gorska MM","Sripada A","Verma D","Vestal B","Sahu A","Moore C","Cambier J","Varma R","Sirohi K","Manka L","Guntur V"],"additional_accession":[]},"is_claimable":false,"name":"Allergens abrogate antiinflammatory DNA effects and unmask macrophage-driven neutrophilic asthma via ILC2/STING/TNF-α signaling.","description":"The mechanisms of neutrophilic and mixed neutrophilic-eosinophilic asthma are poorly understood. We found that extracellular DNA and nucleosomes (Nucs) were elevated in the airways of patients with neutrophilic-eosinophilic asthma and correlated with bronchoalveolar lavage neutrophils. Bronchial tissue from neutrophilic-eosinophilic asthma had more DNA sensor-positive cells. Intranasally administered DNA did not induce airway hyperreactivity (AHR) or any pathology but induced AHR and neutrophilic-eosinophilic inflammation when coadministered with the allergen Alternaria (Alt). Nuc alone induced antiinflammatory/defensive genes, whereas the Nuc-Alt combination increased levels of TNF-α and innate cytokines. The Alt-Nuc phenotype was abolished in Cgas-/-, ALR-/-, Sting-/-, LysMCre:Stingfl/fl","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-05-29T17:57:08.376Z","creation":"2026-05-18T03:07:31.36Z"},"accession":"S-EPMC12352889","cross_references":{"pubmed":["40526428"],"doi":["10.1172/JCI187907"]}}