{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(31)"],"submitter":["Dos Reis Simpronio M"],"pubmed_abstract":["The tumor microenvironment is a complex milieu that has not been properly studied in cells cultured in conventional monolayer. Studies have demonstrated the antitumor activity of epigallocatechin-3-gallate (EGCG), present in green tea, using monolayer cultures without considering the three-dimensional microenvironment of a tumor. Furthermore, many studies have shown the effect of EGCG on the transcriptional profile of cancer cells, but each study has been limited to only one or a few cell types. Using the LINCS database, we characterized the gene signatures produced by EGCG treatment in different cell types and reported a variation in EGCG-induced gene signatures depending on the cell type analyzed. GSEA analysis revealed that EGCG influenced multiple biological pathways related to cell si"],"journal":["ACS omega"],"pagination":["34238-34253"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12355261"],"repository":["biostudies-literature"],"pubmed_title":["Unraveling the Effects of Epigallocatechin-3-gallate on Hepatocellular Carcinoma Cells: A Comparative Analysis of Monolayer vs Multicellular Tumor Spheroids."],"pmcid":["PMC12355261"],"pubmed_authors":["Santos P","Antunes LMG","Aissa AF","Ribeiro DL","Machado ART","Dos Reis Simpronio M"],"additional_accession":[]},"is_claimable":false,"name":"Unraveling the Effects of Epigallocatechin-3-gallate on Hepatocellular Carcinoma Cells: A Comparative Analysis of Monolayer vs Multicellular Tumor Spheroids.","description":"The tumor microenvironment is a complex milieu that has not been properly studied in cells cultured in conventional monolayer. Studies have demonstrated the antitumor activity of epigallocatechin-3-gallate (EGCG), present in green tea, using monolayer cultures without considering the three-dimensional microenvironment of a tumor. Furthermore, many studies have shown the effect of EGCG on the transcriptional profile of cancer cells, but each study has been limited to only one or a few cell types. Using the LINCS database, we characterized the gene signatures produced by EGCG treatment in different cell types and reported a variation in EGCG-induced gene signatures depending on the cell type analyzed. GSEA analysis revealed that EGCG influenced multiple biological pathways related to cell si","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-05-10T04:13:45.185Z","creation":"2026-04-08T01:29:00.043Z"},"accession":"S-EPMC12355261","cross_references":{"pubmed":["40821580"],"doi":["10.1021/acsomega.5c00839"]}}