biostudies-literatureUnknown10(31)Dos Reis Simpronio MThe tumor microenvironment is a complex milieu that has not been properly studied in cells cultured in conventional monolayer. Studies have demonstrated the antitumor activity of epigallocatechin-3-gallate (EGCG), present in green tea, using monolayer cultures without considering the three-dimensional microenvironment of a tumor. Furthermore, many studies have shown the effect of EGCG on the transcriptional profile of cancer cells, but each study has been limited to only one or a few cell types. Using the LINCS database, we characterized the gene signatures produced by EGCG treatment in different cell types and reported a variation in EGCG-induced gene signatures depending on the cell type analyzed. GSEA analysis revealed that EGCG influenced multiple biological pathways related to cell siACS omega34238-34253https://www.ebi.ac.uk/biostudies/studies/S-EPMC12355261biostudies-literatureUnraveling the Effects of Epigallocatechin-3-gallate on Hepatocellular Carcinoma Cells: A Comparative Analysis of Monolayer vs Multicellular Tumor Spheroids.PMC12355261Santos PAntunes LMGAissa AFRibeiro DLMachado ARTDos Reis Simpronio MfalseUnraveling the Effects of Epigallocatechin-3-gallate on Hepatocellular Carcinoma Cells: A Comparative Analysis of Monolayer vs Multicellular Tumor Spheroids.The tumor microenvironment is a complex milieu that has not been properly studied in cells cultured in conventional monolayer. Studies have demonstrated the antitumor activity of epigallocatechin-3-gallate (EGCG), present in green tea, using monolayer cultures without considering the three-dimensional microenvironment of a tumor. Furthermore, many studies have shown the effect of EGCG on the transcriptional profile of cancer cells, but each study has been limited to only one or a few cell types. Using the LINCS database, we characterized the gene signatures produced by EGCG treatment in different cell types and reported a variation in EGCG-induced gene signatures depending on the cell type analyzed. GSEA analysis revealed that EGCG influenced multiple biological pathways related to cell si2025-01-01T00:00:00Z2025 Aug2026-05-10T04:13:45.185Z2026-04-08T01:29:00.043ZS-EPMC123552614082158010.1021/acsomega.5c00839