{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["29"],"submitter":["Xin D"],"pubmed_abstract":["In this study, a process optimization strategy was established to enhance the efficiency of manno-oligosaccharides (MOS) production from spent coffee grounds (SCG), using a sequential approach of aqueous ammonia pretreatment and surfactant-assisted enzymatic hydrolysis. Aqueous ammonia pretreatment (25 % NH₃·H₂O, 70 °C, 48 h) significantly disrupted the lignocellulosic matrix, achieving 67.1 % lignin removal and resulting in more than a threefold increase in MOS yield. The addition of non-ionic surfactants (Tween 80 and PEG 6000) further improved MOS yields by mitigating the non-specific binding of mannanase to lignin. Among them, Tween 80 exhibited superior performance, increasing the MOS yield to 62.3 % at a concentration of 5 mg/mL. This integrated strategy provides a practical and effe"],"journal":["Food chemistry: X"],"pagination":["102892"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12355980"],"repository":["biostudies-literature"],"pubmed_title":["Integrated alkaline pretreatment and surfactant-assisted hydrolysis for high-yield Manno-oligosaccharides production from spent coffee grounds."],"pmcid":["PMC12355980"],"pubmed_authors":["Yin H","Ran G","Xin D","Jiao Z"],"additional_accession":[]},"is_claimable":false,"name":"Integrated alkaline pretreatment and surfactant-assisted hydrolysis for high-yield Manno-oligosaccharides production from spent coffee grounds.","description":"In this study, a process optimization strategy was established to enhance the efficiency of manno-oligosaccharides (MOS) production from spent coffee grounds (SCG), using a sequential approach of aqueous ammonia pretreatment and surfactant-assisted enzymatic hydrolysis. Aqueous ammonia pretreatment (25 % NH₃·H₂O, 70 °C, 48 h) significantly disrupted the lignocellulosic matrix, achieving 67.1 % lignin removal and resulting in more than a threefold increase in MOS yield. The addition of non-ionic surfactants (Tween 80 and PEG 6000) further improved MOS yields by mitigating the non-specific binding of mannanase to lignin. Among them, Tween 80 exhibited superior performance, increasing the MOS yield to 62.3 % at a concentration of 5 mg/mL. This integrated strategy provides a practical and effe","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jul","modification":"2026-04-07T14:37:28.909Z","creation":"2026-04-07T14:12:55.097Z"},"accession":"S-EPMC12355980","cross_references":{"pubmed":["40823139"],"doi":["10.1016/j.fochx.2025.102892"]}}