biostudies-literatureChen LScientific Research Foundation of Wuhan Union HospitalNational Key R&D Program of ChinaNational Natural Science Foundation of ChinaNational Key Research and Development Program of Chinae70447https://www.ebi.ac.uk/biostudies/studies/S-EPMC12356993biostudies-literatureUnknown15(8)<h4>Background</h4>Fucosyltransferase 2 (FUT2)-dependent fucosylation of intestinal epithelial cells is vital for preserving gut barrier integrity and microbial balance. Nevertheless, its precise involvement in alcohol-associated liver disease has yet to be fully elucidated.<h4>Methods</h4>We generated mice with intestinal epithelial cell-specific Fut2 knockout (Fut2^△IEC) and established a chronic-binge alcohol model. 16S rRNA sequencing and metabolomics analysis were used to reveal differences in the composition and function of faecal bacteria.<h4>Results</h4>The loss of intestinal epithelial Fut2 exacerbates alcohol-related hepatic oxidative stress damage, and this effect is dependent on gut bacteria. A marked decrease in the abundance of bacteria carrying nicotinamidase (PncAClinical and translational medicineIntestinal Fut2 deficiency exacerbated alcohol-related liver disease by disrupting nicotinamide metabolism.PMC123569932023XHYN019820005612022YFA13056008197406282400652822743148210037582470584Shang LWang SChen LChu HGao WYuan HHou XYang SYin ZfalseIntestinal Fut2 deficiency exacerbated alcohol-related liver disease by disrupting nicotinamide metabolism.<h4>Background</h4>Fucosyltransferase 2 (FUT2)-dependent fucosylation of intestinal epithelial cells is vital for preserving gut barrier integrity and microbial balance. Nevertheless, its precise involvement in alcohol-associated liver disease has yet to be fully elucidated.<h4>Methods</h4>We generated mice with intestinal epithelial cell-specific Fut2 knockout (Fut2^△IEC) and established a chronic-binge alcohol model. 16S rRNA sequencing and metabolomics analysis were used to reveal differences in the composition and function of faecal bacteria.<h4>Results</h4>The loss of intestinal epithelial Fut2 exacerbates alcohol-related hepatic oxidative stress damage, and this effect is dependent on gut bacteria. A marked decrease in the abundance of bacteria carrying nicotinamidase (PncA2025-01-01T00:00:00Z2025 Aug2026-05-01T18:43:32.261Z2026-04-07T17:09:19.828ZS-EPMC123569934081759810.1002/ctm2.70447