biostudies-literatureUnknown16Chen J<h4>Background</h4>Shenyuan Yiqi Huoxue Capsule (SYYQ) has clinical evidence to improve coronary microvascular dysfunction (CMD) by tonifying qi and removing blood stasis, but the underlying mechanism remains unclear.<h4>Objective</h4>This study aims to explore the mechanism by which SYYQ alleviates CMD through a combination of network analysis and both <i>in vivo</i> and <i>in vitro</i> experiments.<h4>Methods</h4>First, network pharmacology was employed to predict the mechanism of SYYQ on CMD. Building upon the findings of network pharmacology, we conducted <i>in vivo</i> experiment to verify the improvement mechanism of SYYQ in CMD rats using echocardiography, histopathology, serum biochemistry, TUNEL staining, and transmission electron microscopy (TEM). In the context of cell experimenFrontiers in pharmacology1534967https://www.ebi.ac.uk/biostudies/studies/S-EPMC12358463biostudies-literatureMechanism of Shenyuan Yiqi Huoxue capsule alleviating coronary microvascular dysfunction: network analysis and experimental evidence.PMC12358463Liu HLi XLi YChen JZong HZhang SZhou HShi YfalseMechanism of Shenyuan Yiqi Huoxue capsule alleviating coronary microvascular dysfunction: network analysis and experimental evidence.<h4>Background</h4>Shenyuan Yiqi Huoxue Capsule (SYYQ) has clinical evidence to improve coronary microvascular dysfunction (CMD) by tonifying qi and removing blood stasis, but the underlying mechanism remains unclear.<h4>Objective</h4>This study aims to explore the mechanism by which SYYQ alleviates CMD through a combination of network analysis and both <i>in vivo</i> and <i>in vitro</i> experiments.<h4>Methods</h4>First, network pharmacology was employed to predict the mechanism of SYYQ on CMD. Building upon the findings of network pharmacology, we conducted <i>in vivo</i> experiment to verify the improvement mechanism of SYYQ in CMD rats using echocardiography, histopathology, serum biochemistry, TUNEL staining, and transmission electron microscopy (TEM). In the context of cell experimen2025-01-01T00:00:00Z20252026-05-01T01:42:44.152Z2026-04-07T16:33:23.322ZS-EPMC123584634083261210.3389/fphar.2025.1534967