biostudies-literatureBlatnik AJOhio State Pre-accelerator AwardCure SMA FellowshipCenter for RNA BiologyNINDS NIH HHSNIH HHSNIGMS NIH HHSgkaf794https://www.ebi.ac.uk/biostudies/studies/S-EPMC12359035biostudies-literatureUnknown53(15)Sm-ring assembly is important for the biogenesis, stability, and function of uridine-rich small nuclear RNAs (U snRNAs) involved in pre-messenger RNA (mRNA) splicing and histone pre-mRNA processing. Sm-ring assembly is cytoplasmic and dependent upon the Sm-site sequence and structural motif, ATP, and Survival motor neuron (SMN) protein complex. While RNAs other than U snRNAs were previously shown to associate with Sm proteins, whether this association follows Sm-ring assembly requirements is unknown. We systematically identified Sm-sites within the human and mouse transcriptomes and assessed whether these sites can accept Sm-rings. In addition to snRNAs, Sm-sites are highly prevalent in the 3' untranslated regions of long mRNAs. RNA immunoprecipitation experiments confirm that Sm-site contNucleic acids researchSm-site containing mRNAs can accept Sm-rings and are downregulated in Spinal Muscular Atrophy.PMC12359035R35-GM149298R01 NS123736T32-GM141955R35-GM142580T32 GM141955R35 GM1492985R01NS123736-04R35 GM142580Singh GBlatnik AJSlivka JPastore BEmbree CMSanjeev MBurghes AHMTang WfalseSm-site containing mRNAs can accept Sm-rings and are downregulated in Spinal Muscular Atrophy.Sm-ring assembly is important for the biogenesis, stability, and function of uridine-rich small nuclear RNAs (U snRNAs) involved in pre-messenger RNA (mRNA) splicing and histone pre-mRNA processing. Sm-ring assembly is cytoplasmic and dependent upon the Sm-site sequence and structural motif, ATP, and Survival motor neuron (SMN) protein complex. While RNAs other than U snRNAs were previously shown to associate with Sm proteins, whether this association follows Sm-ring assembly requirements is unknown. We systematically identified Sm-sites within the human and mouse transcriptomes and assessed whether these sites can accept Sm-rings. In addition to snRNAs, Sm-sites are highly prevalent in the 3' untranslated regions of long mRNAs. RNA immunoprecipitation experiments confirm that Sm-site cont2025-01-01T00:00:00Z2025 Aug2026-04-08T15:41:09.895Z2026-04-08T05:10:30.654ZS-EPMC123590354082381310.1093/nar/gkaf794