biostudies-literatureWechsler TNational Institute of Neurological Disorders and StrokeNINDS NIH HHSNational Institutes of Health Shared InstrumentationNational Institute of General Medical SciencesNIH HHSNIGMS NIH HHSDNA Technologies and Expression Analysis CoresTraining Program in Molecular and Cellular BiologyNational Science FoundationJapan Society for the Promotion of Sciencegkaf798https://www.ebi.ac.uk/biostudies/studies/S-EPMC12359037biostudies-literatureUnknown53(15)Processing and packaging of messenger ribonucleoprotein (mRNP) particles involve complex, coordinated interactions between nascent transcripts, RNA-binding proteins (RBPs), and associated factors. Despite the critical role of co-transcriptional mRNP assembly in gene expression, the temporal dynamics of this process are not well understood. Here, a live cell imaging assay is reported in Saccharomyces cerevisiae to detect recruitment of endogenous fluorescently tagged proteins to a transcriptionally active locus. Protein recruitment to an inducible integrated gene array composed of 25 transcriptional units is detected by colocalization with lacO repeats. Using arrays with two different promoters and the same coding sequence (GFA1), arrival times for a variety of mRNP processing and assembly Nucleic acids researchA time-resolved framework for the recruitment of mRNP processing and assembly factors to a site of transcription.PMC123590371S10OD010786-01S10 OD010786F31 NS131037T32 GM007377GM007377F31NS1310372140761Concepcion KAsada RDominguez ANMWechsler TMontpetit RMontpetit BMcCormick JKfalseA time-resolved framework for the recruitment of mRNP processing and assembly factors to a site of transcription.Processing and packaging of messenger ribonucleoprotein (mRNP) particles involve complex, coordinated interactions between nascent transcripts, RNA-binding proteins (RBPs), and associated factors. Despite the critical role of co-transcriptional mRNP assembly in gene expression, the temporal dynamics of this process are not well understood. Here, a live cell imaging assay is reported in Saccharomyces cerevisiae to detect recruitment of endogenous fluorescently tagged proteins to a transcriptionally active locus. Protein recruitment to an inducible integrated gene array composed of 25 transcriptional units is detected by colocalization with lacO repeats. Using arrays with two different promoters and the same coding sequence (GFA1), arrival times for a variety of mRNP processing and assembly 2025-01-01T00:00:00Z2025 Aug2026-07-04T03:19:33.202Z2026-07-04T03:13:21.071ZS-EPMC123590374082380810.1093/nar/gkaf798