biostudies-literatureUnknown86Esparza-Molto PBMitochondrial reactive oxygen species (mtROS) regulate cellular signaling pathways, but also cause oxidative stress when de-regulated during aging and pathological conditions such as neurodegenerative diseases. The dynamic redistribution of proteins between cellular compartments is a common mechanism to control their stability and biological activities. By targeting the BirA∗ biotin ligase to the outer mitochondrial membrane in HEK293 cells, we identified proteins whose labeling increased or decreased in response to treatment with menadione, consistent with a dynamic change in their mitochondrial localization in response to increased mtROS production. These proteins represent potential candidates for future studies of mitochondrial oxidative stress signaling. A subset of glycolytic enzymesRedox biology103812https://www.ebi.ac.uk/biostudies/studies/S-EPMC12359183biostudies-literatureROS-dependent localization of glycolytic enzymes to mitochondria.PMC12359183Bozkurt SNewman LEGage FHShadel GSEsparza-Molto PBJones JRGoswami AVGuan DMoyzis AGMunch CRojas GRfalseROS-dependent localization of glycolytic enzymes to mitochondria.Mitochondrial reactive oxygen species (mtROS) regulate cellular signaling pathways, but also cause oxidative stress when de-regulated during aging and pathological conditions such as neurodegenerative diseases. The dynamic redistribution of proteins between cellular compartments is a common mechanism to control their stability and biological activities. By targeting the BirA∗ biotin ligase to the outer mitochondrial membrane in HEK293 cells, we identified proteins whose labeling increased or decreased in response to treatment with menadione, consistent with a dynamic change in their mitochondrial localization in response to increased mtROS production. These proteins represent potential candidates for future studies of mitochondrial oxidative stress signaling. A subset of glycolytic enzymes2025-01-01T00:00:00Z2025 Aug2026-04-08T15:37:44.962Z2026-04-08T05:07:08.454ZS-EPMC123591834080324810.1016/j.redox.2025.103812