biostudies-literaturePark SNational Research Foundation of Koreae70278https://www.ebi.ac.uk/biostudies/studies/S-EPMC12359201biostudies-literatureUnknown34(9)Zinc finger (ZF) proteins are the most abundant transcription factors in vertebrates, and they regulate gene expression through interactions with cis-acting elements. ZF domains selectively recognize specific sequences to accelerate or repress target genes. Zinc finger protein 18 (ZNF18) contains five CX<sub>2</sub>CX<sub>12</sub>HX<sub>3</sub>H-type ZFs at the C-terminus, which are expressed in the brain and other organs of the biological system. Bioinformatic study proposed that cyclin-dependent kinase 1 (CDK1) is in the signaling cascade of ZNF18; although experimental evidence has not yet been reported. In this study, we expressed and purified ZNF18(ZF1-5), five ZF domains from ZNF18, and investigated metal binding specificity and promoter interactions. ZNF18(ZF1-5) has specific coordiProtein science : a publication of the Protein SocietyControlling gene expression through five zinc finger domains of ZNF18.PMC123592012017R1A6A1A03015876Cheong JSLee SJEom KSPark SHwang YfalseControlling gene expression through five zinc finger domains of ZNF18.Zinc finger (ZF) proteins are the most abundant transcription factors in vertebrates, and they regulate gene expression through interactions with cis-acting elements. ZF domains selectively recognize specific sequences to accelerate or repress target genes. Zinc finger protein 18 (ZNF18) contains five CX<sub>2</sub>CX<sub>12</sub>HX<sub>3</sub>H-type ZFs at the C-terminus, which are expressed in the brain and other organs of the biological system. Bioinformatic study proposed that cyclin-dependent kinase 1 (CDK1) is in the signaling cascade of ZNF18; although experimental evidence has not yet been reported. In this study, we expressed and purified ZNF18(ZF1-5), five ZF domains from ZNF18, and investigated metal binding specificity and promoter interactions. ZNF18(ZF1-5) has specific coordi2025-01-01T00:00:00Z2025 Sep2026-05-01T01:39:53.113Z2026-04-07T16:32:51.242ZS-EPMC123592014082392910.1002/pro.70278