biostudies-literatureZhang CNingxia Natural Science FoundationNorth Minzu University826-830https://www.ebi.ac.uk/biostudies/studies/S-EPMC12364091biostudies-literatureUnknown10(9)<i>Oxytropis monophylla</i> belongs to the genus Oxytropis of the family Fabaceae, and is an ecologically significant species, as it plays a vital role in desertification control. However, the chloroplast genome of <i>O. monophylla</i> remains unreported. Here, we constructed a high-quality chloroplast (cp) genome assembly for <i>O. monophylla</i> using NOVOPlasty and Illumina sequencing data. The results showed that the <i>O. monophylla</i> cp genome exhibited atypical structural characteristics due to the absence of an IR region. The chloroplast genome was 122,413 bp long with a GC content of 34.2%, encoding 108 complete genes. Phylogenetic reconstruction through both maximum likelihood and Bayesian inference methods resolved 12 <i>Oxytropis</i> species into two strongly supported cladesMitochondrial DNA. Part B, ResourcesThe complete chloroplast genome of <i>Oxytropis monophylla</i> (Fabaceae): insights into genome features and evolutionary relationships.PMC12364091YCX244092024AAC031602021A1171Ablat ADing XZhao SHu WZhang CfalseThe complete chloroplast genome of <i>Oxytropis monophylla</i> (Fabaceae): insights into genome features and evolutionary relationships.<i>Oxytropis monophylla</i> belongs to the genus Oxytropis of the family Fabaceae, and is an ecologically significant species, as it plays a vital role in desertification control. However, the chloroplast genome of <i>O. monophylla</i> remains unreported. Here, we constructed a high-quality chloroplast (cp) genome assembly for <i>O. monophylla</i> using NOVOPlasty and Illumina sequencing data. The results showed that the <i>O. monophylla</i> cp genome exhibited atypical structural characteristics due to the absence of an IR region. The chloroplast genome was 122,413 bp long with a GC content of 34.2%, encoding 108 complete genes. Phylogenetic reconstruction through both maximum likelihood and Bayesian inference methods resolved 12 <i>Oxytropis</i> species into two strongly supported clades2025-01-01T00:00:00Z20252026-05-29T14:45:11.022Z2026-04-08T05:11:25.01ZS-EPMC123640914083725210.1080/23802359.2025.2548846