biostudies-literaturePiontkivska DPrograma Operacional Regional de Lisboa 2020U.S. Department of EnergyFundação para a Ciência e a TecnologiaU.S. Department of Energy, Office of Science, Biological and Environmental Research Division,European Regional Development Funde70153https://www.ebi.ac.uk/biostudies/studies/S-EPMC12365344biostudies-literatureUnknown17(4)Bacterial-fungal interactions that influence the behaviour of one or both organisms are common in nature. Well-studied systems include endosymbiotic relationships that range from transient to long-term associations. Diverse endohyphal bacteria associate with fungal hosts, emphasising the need to better comprehend the fungal bacteriome. We evaluated the hypothesis that Aspergillus fumigatus harbours an endohyphal community of bacteria that influence the host phenotype. We analysed whether 38 A. fumigatus strains show stable association with diverse endohyphal bacteria; all derived from single-conidium cultures that were subjected to antibiotic and heat treatments. The fungal bacteriome, inferred through analysis of bacterial diversity within the fungal strains (short- and long- read sequencEnvironmental microbiology reportsHidden Allies: Decoding the Core Endohyphal Bacteriome of Aspergillus fumigatus.PMC12365344PTDC/CTA‐AMB/6587/20202023ARA0025PD/BD/138913/2018PPBI-POCI-01-0145-FEDER-022122LA/P/0140/2020UIDB/04612/2020, UIDP/04612/2020PPBI‐POCI‐01‐0145‐FEDER‐022122LANLF59TUID/BIO/04565/2020LA/P/0087/2020PTDC/CTA-AMB/6587/2020Project N. 007317Silva Pereira CGoldman GHPiontkivska DMorales DPJorge JMPCrespo PMartins TMCarvalho DMelo-Cristino JMil-Homens DSa-Leao RfalseHidden Allies: Decoding the Core Endohyphal Bacteriome of Aspergillus fumigatus.Bacterial-fungal interactions that influence the behaviour of one or both organisms are common in nature. Well-studied systems include endosymbiotic relationships that range from transient to long-term associations. Diverse endohyphal bacteria associate with fungal hosts, emphasising the need to better comprehend the fungal bacteriome. We evaluated the hypothesis that Aspergillus fumigatus harbours an endohyphal community of bacteria that influence the host phenotype. We analysed whether 38 A. fumigatus strains show stable association with diverse endohyphal bacteria; all derived from single-conidium cultures that were subjected to antibiotic and heat treatments. The fungal bacteriome, inferred through analysis of bacterial diversity within the fungal strains (short- and long- read sequenc2025-01-01T00:00:00Z2025 Aug2026-05-29T17:06:17.002Z2026-04-08T05:25:25.137ZS-EPMC123653444083118910.1111/1758-2229.70153