{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Givens SE"],"funding":["University of Minnesota","National Heart Lung and Blood Institute Division of Intramural Research","National Science Foundation"],"pagination":["102572"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12365841"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(8)"],"pubmed_abstract":["Accumulating evidence indicates that maturation limits cardiomyocyte proliferation. We expand on that theory by co-culturing human induced pluripotent stem cell (hiPSC)-cardiomyocytes (CM) with epicardial cells (EPCs) and epicardial-derived cells in both 2D co-cultures and 3D engineered heart tissues (EHTs). In 2D co-cultures, the percentage of proliferating CM increased in parallel with stark electrophysiologic improvements. Single-cell transcriptomics revealed a significant shift in the bulk CM population of the epicardial-CM co-cultures as characterized by more fetal-like myofilament isoforms but with enhanced pathways associated with electrochemical maturation. The 3D-EHTs containing EPCs showed more limited proliferation but a similar improvement in CM electrophysiologic function. Nex"],"journal":["Stem cell reports"],"pubmed_title":["Developmental cues from epicardial cells simultaneously promote cardiomyocyte proliferation and electrochemical maturation."],"pmcid":["PMC12365841"],"funding_grant_id":["GRFP 2019272039"],"pubmed_authors":["Rothermel TM","Andebrhan AA","Ebrahimi-Barough S","Johnson M","Hosseini S","Givens SE","Dudley S","Torniainen AA","Boland SF","Singh BN","Mancipe NC","Wang R","Ogle BM","Alford PW","Xie A","Shameem M","Kong X","Tolkacheva EG","van Berlo JH"],"additional_accession":[]},"is_claimable":false,"name":"Developmental cues from epicardial cells simultaneously promote cardiomyocyte proliferation and electrochemical maturation.","description":"Accumulating evidence indicates that maturation limits cardiomyocyte proliferation. We expand on that theory by co-culturing human induced pluripotent stem cell (hiPSC)-cardiomyocytes (CM) with epicardial cells (EPCs) and epicardial-derived cells in both 2D co-cultures and 3D engineered heart tissues (EHTs). In 2D co-cultures, the percentage of proliferating CM increased in parallel with stark electrophysiologic improvements. Single-cell transcriptomics revealed a significant shift in the bulk CM population of the epicardial-CM co-cultures as characterized by more fetal-like myofilament isoforms but with enhanced pathways associated with electrochemical maturation. The 3D-EHTs containing EPCs showed more limited proliferation but a similar improvement in CM electrophysiologic function. Nex","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-05-29T14:42:46.77Z","creation":"2026-04-08T05:07:07.087Z"},"accession":"S-EPMC12365841","cross_references":{"pubmed":["40614731"],"doi":["10.1016/j.stemcr.2025.102572"]}}