{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bao G"],"funding":["Yunnan Provincial Fund for High-Level Reserve Talents in Health Science","Yunnan Applied Basic Research Projects-Kunming Medical University Union Foundation","National Natural Science Foundation of China"],"pagination":["279"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12366207"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["31(1)"],"pubmed_abstract":["Background
Disruption in bile duct barrier function contributes to hepatocyte toxicity in ischemia-reperfusion injury, often leading to surgical complications in liver resection, transplantation, and hemorrhagic shock. However, the underlying mechanisms remain incompletely understood.Methods
Transcriptomic and proteomic analyses were conducted to examine tryptophan (Trp) metabolism in a Pringle maneuver-induced bile duct injury rat model; Hypoxia/Reoxygenation (H/R) was used to establish an in vitro cholangiocyte injury model. Cholangiocyte injury was assessed via hematoxylin and eosin (H&E) staining, Ki67/myeloperoxidase (MPO) immunohistochemistry, transmission electron microscopy (TEM), and TUNEL/CK19 co-staining. Tight junction integrity was evaluated by measuring transe"],"journal":["Molecular medicine (Cambridge, Mass.)"],"pubmed_title":["Kynu inhibition mitigates bile duct ischemic injury by rewiring tryptophan metabolism to restore tight junction integrity."],"pmcid":["PMC12366207"],"funding_grant_id":["202001AY070001-040","81860121","H-2018068"],"pubmed_authors":["Bao G","Bi P","Yang B","Zhang S","Ye Z","Luo D"],"additional_accession":[]},"is_claimable":false,"name":"Kynu inhibition mitigates bile duct ischemic injury by rewiring tryptophan metabolism to restore tight junction integrity.","description":"Background
Disruption in bile duct barrier function contributes to hepatocyte toxicity in ischemia-reperfusion injury, often leading to surgical complications in liver resection, transplantation, and hemorrhagic shock. However, the underlying mechanisms remain incompletely understood.Methods
Transcriptomic and proteomic analyses were conducted to examine tryptophan (Trp) metabolism in a Pringle maneuver-induced bile duct injury rat model; Hypoxia/Reoxygenation (H/R) was used to establish an in vitro cholangiocyte injury model. Cholangiocyte injury was assessed via hematoxylin and eosin (H&E) staining, Ki67/myeloperoxidase (MPO) immunohistochemistry, transmission electron microscopy (TEM), and TUNEL/CK19 co-staining. Tight junction integrity was evaluated by measuring transe","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-05-05T18:04:56.18Z","creation":"2026-04-07T21:43:47.947Z"},"accession":"S-EPMC12366207","cross_references":{"pubmed":["40830424"],"doi":["10.1186/s10020-025-01310-6"]}}