biostudies-literatureWelters KDeutsche Forschungsgemeinschaft694-701https://www.ebi.ac.uk/biostudies/studies/S-EPMC12366286biostudies-literatureUnknown46(11-12)We present the coupling of capillary electrophoresis to a custom-built high-resolution ion mobility spectrometer (IMS). This system integrates a shifted inlet potential IMS configuration with a customised nanoflow ESI sheath interface. It enables the rapid analysis of quaternary ammonium compounds (QACs) and their impurities in real-world samples. It allowed the detection of six non-chromophoric compounds in about 3 min. The assignment of the IMS signals to compounds was supported by matching experimentally determined collision cross-section (CCS) values with predicted values. The system achieved a detection limit in the single-digit picogram range with IMS resolutions of over 80.ElectrophoresisCoupling Capillary Electrophoresis With a Shifted Inlet Potential High-Resolution Ion Mobility Spectrometer.PMC12366286458829155Schlottmann FZimmermann SBelder DThoben CWelters KRaddatz CRfalseCoupling Capillary Electrophoresis With a Shifted Inlet Potential High-Resolution Ion Mobility Spectrometer.We present the coupling of capillary electrophoresis to a custom-built high-resolution ion mobility spectrometer (IMS). This system integrates a shifted inlet potential IMS configuration with a customised nanoflow ESI sheath interface. It enables the rapid analysis of quaternary ammonium compounds (QACs) and their impurities in real-world samples. It allowed the detection of six non-chromophoric compounds in about 3 min. The assignment of the IMS signals to compounds was supported by matching experimentally determined collision cross-section (CCS) values with predicted values. The system achieved a detection limit in the single-digit picogram range with IMS resolutions of over 80.2025-01-01T00:00:00Z2025 Jun2026-05-05T12:35:36.089Z2026-04-07T21:42:16.674ZS-EPMC123662864029285010.1002/elps.8147