{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jeong H"],"funding":["National Human Genome Research Institute of the National Institutes of Health","NHGRI NIH HHS"],"pagination":["e2504687"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12366287"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["21(32)"],"pubmed_abstract":["Studies on the dynamics of single cell phenotyping have been hampered by the lack of quantitative high-throughput metabolism assays. Extracellular acidification, a prominent phenotype, yields significant insights into cellular metabolism, including tumorigenicity. Here, it is developed a versatile microfluidic system for single cell optical pH analysis (SCO-pH), which compartmentalizes single cells in 140-pL droplets and immobilizes ≈40,000 droplets in a 2D array for temporal extracellular pH analysis. SCO-pH distinguishes cells undergoing hyperglycolysis induced by oligomycin A from untreated cells by monitoring their extracellular acidification. To facilitate pH sensing in each droplet, a cell-impermeable pH probe is encapsulated and its fluorescence intensities are quantified. Using this approach, hyperglycolytic cells can be differentiated, and single-cell heterogeneity in extracellular acidification dynamics can be concurrently observed. This high-throughput system will be useful in applications that require dynamic phenotyping of single cells with significant heterogeneity."],"journal":["Small (Weinheim an der Bergstrasse, Germany)"],"pubmed_title":["SCO-ph: Microfluidic Dynamic Phenotyping Platform for High-Throughput Screening of Single Cell Acidification."],"pmcid":["PMC12366287"],"funding_grant_id":["RM1HG010023"],"pubmed_authors":["Kuang D","Sul JY","Leyes Porello EA","Lim B","Jeong H","Han SH","Rosario JG","Kim J","Lee D"],"additional_accession":[]},"is_claimable":false,"name":"SCO-ph: Microfluidic Dynamic Phenotyping Platform for High-Throughput Screening of Single Cell Acidification.","description":"Studies on the dynamics of single cell phenotyping have been hampered by the lack of quantitative high-throughput metabolism assays. Extracellular acidification, a prominent phenotype, yields significant insights into cellular metabolism, including tumorigenicity. Here, it is developed a versatile microfluidic system for single cell optical pH analysis (SCO-pH), which compartmentalizes single cells in 140-pL droplets and immobilizes ≈40,000 droplets in a 2D array for temporal extracellular pH analysis. SCO-pH distinguishes cells undergoing hyperglycolysis induced by oligomycin A from untreated cells by monitoring their extracellular acidification. To facilitate pH sensing in each droplet, a cell-impermeable pH probe is encapsulated and its fluorescence intensities are quantified. Using this approach, hyperglycolytic cells can be differentiated, and single-cell heterogeneity in extracellular acidification dynamics can be concurrently observed. This high-throughput system will be useful in applications that require dynamic phenotyping of single cells with significant heterogeneity.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-05-05T12:39:31.607Z","creation":"2026-04-26T03:06:15.269Z"},"accession":"S-EPMC12366287","cross_references":{"pubmed":["40519058"],"doi":["10.1002/smll.202504687"]}}