{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16(1)"],"submitter":["Steger M"],"pubmed_abstract":["Molecular glue degraders (MGDs) are small molecules that co-opt the ubiquitin-proteasome system to induce degradation of target proteins, including those considered undruggable. Their discovery remains challenging due to the lack of rational design strategies and limited throughput of unbiased proteome-wide screening approaches. To address this gap, we develop a high-throughput proteomics platform based on label-free, data-independent acquisition mass spectrometry (DIA-MS), enabling integrated proteomics and ubiquitinomics profiling. Screening a diverse set of 100 cereblon (CRBN)-recruiting ligands on this platform leads to identification of a broad array of novel degraders and neosubstrates. Subsequent hit validation and structure-degradation relationship analyses guided by global proteom"],"journal":["Nature communications"],"pagination":["7773"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12368047"],"repository":["biostudies-literature"],"pubmed_title":["Unbiased mapping of cereblon neosubstrate landscape by high-throughput proteomics."],"pmcid":["PMC12368047"],"pubmed_authors":["Demichev V","Price J","Graef T","Yang J","Nishiguchi G","Yang L","Daub H","Wu Q","Bartoschek D","Shi Z","Rankovic Z","Mayasundari A","Ohmayer U","Actis M","Steger M","Aggarwal A","McGowan K","Schwalb B","Shashikadze B","Bednarz AH","Machata S"],"additional_accession":[]},"is_claimable":false,"name":"Unbiased mapping of cereblon neosubstrate landscape by high-throughput proteomics.","description":"Molecular glue degraders (MGDs) are small molecules that co-opt the ubiquitin-proteasome system to induce degradation of target proteins, including those considered undruggable. Their discovery remains challenging due to the lack of rational design strategies and limited throughput of unbiased proteome-wide screening approaches. To address this gap, we develop a high-throughput proteomics platform based on label-free, data-independent acquisition mass spectrometry (DIA-MS), enabling integrated proteomics and ubiquitinomics profiling. Screening a diverse set of 100 cereblon (CRBN)-recruiting ligands on this platform leads to identification of a broad array of novel degraders and neosubstrates. Subsequent hit validation and structure-degradation relationship analyses guided by global proteom","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-05-05T19:03:57.68Z","creation":"2026-04-07T21:51:08.908Z"},"accession":"S-EPMC12368047","cross_references":{"pubmed":["40835825"],"doi":["10.1038/s41467-025-62829-0"]}}