{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16(1)"],"submitter":["Huang Q"],"funding":["Changping Laboratory"],"pubmed_abstract":["Stabilizing the RSV F protein in its prefusion conformation is crucial for effective vaccine development but has remained a significant challenge. Traditional stabilization methods, such as disulfide bonds and cavity-filling mutations, have been labor-intensive and have often resulted in suboptimal expression levels. Here, we report the design of an RSV prefusion F (preF) antigen using a proline-scanning strategy, incorporating seven proline substitutions to achieve stabilization. The resulting variant, preF7P, is structurally and biochemically validated to maintain the correct prefusion state. PreF7P demonstrates superior immunogenicity with a 1.8-fold increase in neutralizing antibody titers when compared to DS-cav2, and provides protection from clinical disease against both RSV A and B "],"journal":["Nature communications"],"pagination":["7805"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12370985"],"repository":["biostudies-literature"],"pubmed_title":["Highly scalable prefusion-stabilized RSV F vaccine with enhanced immunogenicity and robust protection."],"pmcid":["PMC12370985"],"pubmed_authors":["Yan J","Lang Q","Wang F","Han X","Huang Q","Bai Y","Gao C","Li Y","Zhao L","Cao X","Bian L","Luo L","Wang Y","Duan X","Song Y","Xu Y"],"additional_accession":[]},"is_claimable":false,"name":"Highly scalable prefusion-stabilized RSV F vaccine with enhanced immunogenicity and robust protection.","description":"Stabilizing the RSV F protein in its prefusion conformation is crucial for effective vaccine development but has remained a significant challenge. Traditional stabilization methods, such as disulfide bonds and cavity-filling mutations, have been labor-intensive and have often resulted in suboptimal expression levels. Here, we report the design of an RSV prefusion F (preF) antigen using a proline-scanning strategy, incorporating seven proline substitutions to achieve stabilization. The resulting variant, preF7P, is structurally and biochemically validated to maintain the correct prefusion state. PreF7P demonstrates superior immunogenicity with a 1.8-fold increase in neutralizing antibody titers when compared to DS-cav2, and provides protection from clinical disease against both RSV A and B ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-05-08T10:52:10.894Z","creation":"2026-05-03T03:06:03.485Z"},"accession":"S-EPMC12370985","cross_references":{"pubmed":["40841372"],"doi":["10.1038/s41467-025-63084-z"]}}