{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Horii Y"],"funding":["Japan Society for the Promotion of Science"],"pagination":["30765"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12371019"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["IL-18 is a unique cytokine that exerts both pro- and anti-inflammatory effects depending on the surrounding environments. Excessive inflammation in the feto-maternal interface is thought to result in the onset of miscarriage and preterm birth, but much is unknown about the function of IL-18 in pregnancy. Here, we report the protective role of IL-18 in pregnancy using lipopolysaccharide (LPS)-induced murine miscarriage models. Whereas a low dose (1 µg) of LPS injection in pregnant mice did not induce miscarriages, the additional injection of anti-IL-18 neutralizing antibody (IL-18 nAb) resulted in a significant increase in abortion rates among the pregnant mice. Under these conditions, multiple T-cell subsets and natural killer cells produced significantly lower levels of the type 1 cytokine IFN-γ and the type 2 cytokine IL-4. The miscarriage induced by the low-dose LPS along with the IL-18 nAb was improved by the supplementation of IFN-γ and IL-4. Furthermore, the main source of IL-18 production was found to be the myometrium rather than macrophages. These results indicated that IL-18, which was initially thought to have a harmful effect on the maintenance of pregnancy, has a protective role in miscarriage against minor stimulation by pathogens with modulating immune homeostasis."],"journal":["Scientific reports"],"pubmed_title":["Inflammatory cytokine interleukin-18 prevents murine miscarriage by inducing appropriate inflammation."],"pmcid":["PMC12371019"],"funding_grant_id":["20K09679","22K11505","16H05178"],"pubmed_authors":["Negishi Y","Suzuki S","Koike E","Sasaki F","Horii Y","Flavell RA","Ino H","Kawamoto Y","Ishibashi M","Morita R","Ohashi R"],"additional_accession":[]},"is_claimable":false,"name":"Inflammatory cytokine interleukin-18 prevents murine miscarriage by inducing appropriate inflammation.","description":"IL-18 is a unique cytokine that exerts both pro- and anti-inflammatory effects depending on the surrounding environments. Excessive inflammation in the feto-maternal interface is thought to result in the onset of miscarriage and preterm birth, but much is unknown about the function of IL-18 in pregnancy. Here, we report the protective role of IL-18 in pregnancy using lipopolysaccharide (LPS)-induced murine miscarriage models. Whereas a low dose (1 µg) of LPS injection in pregnant mice did not induce miscarriages, the additional injection of anti-IL-18 neutralizing antibody (IL-18 nAb) resulted in a significant increase in abortion rates among the pregnant mice. Under these conditions, multiple T-cell subsets and natural killer cells produced significantly lower levels of the type 1 cytokine IFN-γ and the type 2 cytokine IL-4. The miscarriage induced by the low-dose LPS along with the IL-18 nAb was improved by the supplementation of IFN-γ and IL-4. Furthermore, the main source of IL-18 production was found to be the myometrium rather than macrophages. These results indicated that IL-18, which was initially thought to have a harmful effect on the maintenance of pregnancy, has a protective role in miscarriage against minor stimulation by pathogens with modulating immune homeostasis.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-05-09T10:46:50.249Z","creation":"2026-04-08T00:48:57.618Z"},"accession":"S-EPMC12371019","cross_references":{"pubmed":["40841464"],"doi":["10.1038/s41598-025-16546-9"]}}