{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kim E"],"funding":["NIDA NIH HHS","Whitehall Foundation","NIMH NIH HHS","U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS)","U.S. Department of Health &amp; Human Services | NIH | National Institute on Drug Abuse","Brain and Behavior Research Foundation","U.S. Department of Health &amp; Human Services | NIH | National Institute of Mental Health","Brain Research Foundation (BRF)","Whitehall Foundation (Whitehall Foundation, Inc.)","U.S. Department of Health &amp; Human Services | NIH | National Institute of Neurological Disorders and Stroke","Brain Research Foundation","Brain and Behavior Research Foundation (Brain & Behavior Research Foundation)","U.S. Department of Health & Human Services | NIH | National Institute on Drug Abuse (NIDA)","NINDS NIH HHS"],"pagination":["7819"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12371101"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(1)"],"pubmed_abstract":["Accumulating evidence has supported diverse regulatory functions of astrocytes in different neural circuits as well as various aspects of complex behaviors. However, little is known about how astrocytes regulate different neuronal subpopulations that are linked to specific behavioral aspects within a single brain region. Here, we show that astrocytes in the medial prefrontal cortex (mPFC) encode anxiogenic environmental cues in freely behaving mice. Silencing mPFC astrocyte Ca<sup>2+</sup> signaling heightens anxiety-like behavior and triggers opposing functional responses in excitatory and inhibitory neurons. Moreover, neuronal subpopulations tuned to anxiety-like behavior are differentially modulated by mPFC astrocytes at single cell and network levels. Using cell type-specific proximity biotinylation approaches, we identified significant intracellular and intercellular proteomic alterations in mPFC astrocytes and at the astrocyte-neuron interface associated with anxiety. Collectively, our findings uncover mechanisms underpinning the heterogenous astrocyte-neuron interaction that is behaviorally relevant and offer critical insights into the pathophysiology of emotional disorders."],"journal":["Nature communications"],"pubmed_title":["Prefrontal cortex astrocytes modulate distinct neuronal populations to control anxiety-like behavior."],"pmcid":["PMC12371101"],"funding_grant_id":["BRFSG-2021-12","DP2 NS136871D","DP2 NS136871","30748","P30 DA018310","2021-08-025","P30DA018310","R01 MH132556"],"pubmed_authors":["Kim E","Tan Y","Diaz-Castro B","Du H","Brown BL","Chang Y","Sweedler JV","Yu X"],"additional_accession":[]},"is_claimable":false,"name":"Prefrontal cortex astrocytes modulate distinct neuronal populations to control anxiety-like behavior.","description":"Accumulating evidence has supported diverse regulatory functions of astrocytes in different neural circuits as well as various aspects of complex behaviors. However, little is known about how astrocytes regulate different neuronal subpopulations that are linked to specific behavioral aspects within a single brain region. Here, we show that astrocytes in the medial prefrontal cortex (mPFC) encode anxiogenic environmental cues in freely behaving mice. Silencing mPFC astrocyte Ca<sup>2+</sup> signaling heightens anxiety-like behavior and triggers opposing functional responses in excitatory and inhibitory neurons. Moreover, neuronal subpopulations tuned to anxiety-like behavior are differentially modulated by mPFC astrocytes at single cell and network levels. Using cell type-specific proximity biotinylation approaches, we identified significant intracellular and intercellular proteomic alterations in mPFC astrocytes and at the astrocyte-neuron interface associated with anxiety. Collectively, our findings uncover mechanisms underpinning the heterogenous astrocyte-neuron interaction that is behaviorally relevant and offer critical insights into the pathophysiology of emotional disorders.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-07-05T03:15:13.671Z","creation":"2026-07-05T03:12:08.929Z"},"accession":"S-EPMC12371101","cross_references":{"pubmed":["40841557"],"doi":["10.1038/s41467-025-63131-9"]}}