{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Prasad PK"],"funding":["Israel Science Foundation","Minerva Foundation"],"pagination":["e2411730"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12372432"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["21(33)"],"pubmed_abstract":["Turn-on fluorescent probes consisting of dye-ligand conjugates serve as a powerful tool for detecting cell surface proteins (CSPs) and their interactions with binding partners. However, generating such probes from protein-based ligands remains challenging. This challenge became particularly evident during the COVID-19 pandemic, which highlighted the need for assays to evaluate inhibitors of the interaction between the SARS-CoV-2 virus receptor-binding domain (RBD) and the angiotensin-converting enzyme 2 (ACE2) receptor. To sense this interaction in a cellular environment using turn-on probes, a tri-nitrilotriacetic acid (tri-NTA) unit was conjugated to quinoline-based cyanine (QBC) dyes. This design leverages the high affinity of tri-NTA for His-tag, along with the low-background and confi"],"journal":["Small (Weinheim an der Bergstrasse, Germany)"],"pubmed_title":["Low-Background His-Tag-Targeting Probes for Turn-On Fluorescence Detection of Cell Surface Proteins and Their Binding Interactions."],"pmcid":["PMC12372432"],"funding_grant_id":["714437","304/22"],"pubmed_authors":["Oppenheimer-Low N","Prasad PK","Vishweshwara SS","Avinoam O","Anand S","Shanthamurthy CD","Kikkeri R","Unger T","Albeck S","Toraskar S","Sirkis YF","Motiei L","Margulies D","Petrovich-Kopitman E","Granot T","Addadi Y","Raigawali R","Yardeni EH","Khan S"],"additional_accession":[]},"is_claimable":false,"name":"Low-Background His-Tag-Targeting Probes for Turn-On Fluorescence Detection of Cell Surface Proteins and Their Binding Interactions.","description":"Turn-on fluorescent probes consisting of dye-ligand conjugates serve as a powerful tool for detecting cell surface proteins (CSPs) and their interactions with binding partners. However, generating such probes from protein-based ligands remains challenging. This challenge became particularly evident during the COVID-19 pandemic, which highlighted the need for assays to evaluate inhibitors of the interaction between the SARS-CoV-2 virus receptor-binding domain (RBD) and the angiotensin-converting enzyme 2 (ACE2) receptor. To sense this interaction in a cellular environment using turn-on probes, a tri-nitrilotriacetic acid (tri-NTA) unit was conjugated to quinoline-based cyanine (QBC) dyes. This design leverages the high affinity of tri-NTA for His-tag, along with the low-background and confi","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-05-08T06:49:16.218Z","creation":"2026-04-07T23:31:14.07Z"},"accession":"S-EPMC12372432","cross_references":{"pubmed":["40613236"],"doi":["10.1002/smll.202411730"]}}