biostudies-literatureBenderski KEuropean Research CouncilDeutsche Forschungsgemeinschaft (German Research Foundation)1163-1172https://www.ebi.ac.uk/biostudies/studies/S-EPMC12373501biostudies-literatureUnknown20(8)Multi-drug nanomedicine is gaining momentum for co-delivering more than one drug to the same site at the same time. Our analysis of 273 pre-clinical tumour growth inhibition studies shows that multi-drug nanotherapy outperforms single-drug therapy, multi-drug combination therapy, and single-drug nanotherapy by 43, 29 and 30%, respectively. Combination nanotherapy also results in the best overall survival rates, with 56% of studies demonstrating complete or partial survival, versus 20-37% for control regimens. Within the multi-drug nanomedicine groups, we analysed the effect of (co-)administration schedule and strategy, passive versus active targeting, nanocarrier material and the type of therapeutic agent. Most importantly, it was found that co-encapsulating two different drugs in the sameNature nanotechnologyAnalysis of multi-drug cancer nanomedicine.PMC12373501KFO344864121SFB1066Benderski KLammers TSofias AMfalseAnalysis of multi-drug cancer nanomedicine.Multi-drug nanomedicine is gaining momentum for co-delivering more than one drug to the same site at the same time. Our analysis of 273 pre-clinical tumour growth inhibition studies shows that multi-drug nanotherapy outperforms single-drug therapy, multi-drug combination therapy, and single-drug nanotherapy by 43, 29 and 30%, respectively. Combination nanotherapy also results in the best overall survival rates, with 56% of studies demonstrating complete or partial survival, versus 20-37% for control regimens. Within the multi-drug nanomedicine groups, we analysed the effect of (co-)administration schedule and strategy, passive versus active targeting, nanocarrier material and the type of therapeutic agent. Most importantly, it was found that co-encapsulating two different drugs in the same2025-01-01T00:00:00Z2025 Aug2026-05-09T10:39:38.614Z2026-04-08T00:48:19.152ZS-EPMC123735014037479610.1038/s41565-025-01932-1