biostudies-literatureWu Zthe Science and Technology Planning Project of Shenzhen Municipality, Chinathe Shenzhen Key Medical Disciplinethe National Natural Science Foundation of China1595https://www.ebi.ac.uk/biostudies/studies/S-EPMC12373589biostudies-literatureUnknown16(1)Nasopharyngeal carcinoma (NPC) is a malignant tumor with high incidence in Southeast Asia and Southern China, characterized by difficulties in early diagnosis and high recurrence rates after treatment. Metabolic reprogramming plays a crucial role in the development and progression of tumors. In-depth studies on the metabolic characteristics and molecular mechanisms of NPC are essential to identify novel diagnostic and therapeutic targets. This study aimed to systematically reveal the metabolic characteristics and molecular mechanisms of NPC cell lines by integrating untargeted metabolomics, transcriptomics, and confocal micro-Raman spectroscopy (CMRS), and to explore potential biomarkers for prognostic evaluation and precision treatment of NPC. We performed an integrated analysis of transcDiscover oncologyKYNU is a potential metabolic-related biomarker for nasopharyngeal carcinoma by Raman spectroscopy, metabolomics, and transcriptomics analysis.PMC12373589No. 81772921JCYJ20230807142806014No. SZXK054Yang HWu ZLi XJi XZhang XLiang MXiong DZhang CXu YChen DfalseKYNU is a potential metabolic-related biomarker for nasopharyngeal carcinoma by Raman spectroscopy, metabolomics, and transcriptomics analysis.Nasopharyngeal carcinoma (NPC) is a malignant tumor with high incidence in Southeast Asia and Southern China, characterized by difficulties in early diagnosis and high recurrence rates after treatment. Metabolic reprogramming plays a crucial role in the development and progression of tumors. In-depth studies on the metabolic characteristics and molecular mechanisms of NPC are essential to identify novel diagnostic and therapeutic targets. This study aimed to systematically reveal the metabolic characteristics and molecular mechanisms of NPC cell lines by integrating untargeted metabolomics, transcriptomics, and confocal micro-Raman spectroscopy (CMRS), and to explore potential biomarkers for prognostic evaluation and precision treatment of NPC. We performed an integrated analysis of transc2025-01-01T00:00:00Z2025 Aug2026-05-08T10:48:49.887Z2026-04-07T23:48:14.707ZS-EPMC123735894084468210.1007/s12672-025-03349-7