biostudies-literaturede Morais Valentim JMBFundação Parque Tecnológico ItaipuConselho Nacional de Desenvolvimento Científico e TecnológicoSecretário de Ciência, Tecnologia e Ensino Superior, Governo do Estado de Parana30910https://www.ebi.ac.uk/biostudies/studies/S-EPMC12373925biostudies-literatureUnknown15(1)Rural women are at a higher risk of developing breast cancer, but the molecular mechanisms involved remain poorly understood. This study investigates the impact of chronic pesticide exposure on systemic and tissue-level protein expressions in women diagnosed with breast cancer. Plasma and tumor samples were collected from 215 women diagnosed with breast cancer; among them, 128 patients were occupationally exposed (n = 128) and 87 patients were not occupationally exposed (87) were analyzed for further studies. High-throughput proteomic analyses of plasma revealed distinct protein expression profiles between the groups, primarily associated with inflammation, oxidative stress, and immune deregulation. Validation analysis showed that cytokines such as IL-1β, IL-12, IL-17A, and TNF-α were signScientific reportsPesticide exposome reveals oxidative stress and immune response disruptions in occupationally exposed women with breast cancer.PMC123739254500007500/2024220/2024402364/2021-0, 441017/2023-1, and 305335/2021-9Pizzatti LKern RSilveira GFDos Santos MLFBordignon JSeiva FRFde Morais Valentim JMBFoti LPavanelli WRPanis CCoradi CIago Oliveira Coletto MRech DFadel BVacario BGLCristina Cazagranda IGaboardi SCCataneo AHDfalsePesticide exposome reveals oxidative stress and immune response disruptions in occupationally exposed women with breast cancer.Rural women are at a higher risk of developing breast cancer, but the molecular mechanisms involved remain poorly understood. This study investigates the impact of chronic pesticide exposure on systemic and tissue-level protein expressions in women diagnosed with breast cancer. Plasma and tumor samples were collected from 215 women diagnosed with breast cancer; among them, 128 patients were occupationally exposed (n = 128) and 87 patients were not occupationally exposed (87) were analyzed for further studies. High-throughput proteomic analyses of plasma revealed distinct protein expression profiles between the groups, primarily associated with inflammation, oxidative stress, and immune deregulation. Validation analysis showed that cytokines such as IL-1β, IL-12, IL-17A, and TNF-α were sign2025-01-01T00:00:00Z2025 Aug2026-04-08T09:14:31.781Z2026-04-08T00:48:07.5ZS-EPMC123739254084671910.1038/s41598-025-12870-2