biostudies-literatureGong JExploration of the mechanism by which Longhu Xingnao decoction alleviates CIRI via NUDT1-mediated m7G methylation modification of GPX4 inhibition of neuronal ferroptosis30953https://www.ebi.ac.uk/biostudies/studies/S-EPMC12373990biostudies-literatureUnknown15(1)Intracerebral hemorrhage (ICH) is the most morbid of all stroke types with a high mortality and significant disability burden. Inhibiting ferroptosis after ICH will effectively treat ICH and improve prognosis. Esculetin is the main active ingredient of the Chinese medicine Cortex Fraxini, which has neuroprotective effects. 7-methylguanosine (m7G) methylation is a common RNA modification that regulates mRNA stability and translation, playing an important role in neural development and function, as well as in ferroptosis-related pathways. In this study, we used hemin-induced PC12 cells to investigate the impact of esculetin on ferroptosis and the involvement of m7G modification. Cell viability was measured by cell counting kit-8. Cell death was evaluated by measuring the levels of LDH releasScientific reportsEsculetin inhibits ferroptosis after ICH by promoting NUDT1-mediated m7G methylation modification of GPX4.PMC123739902025JJ809502025-2027Zhang ZMei ZGong JLi LChen TfalseEsculetin inhibits ferroptosis after ICH by promoting NUDT1-mediated m7G methylation modification of GPX4.Intracerebral hemorrhage (ICH) is the most morbid of all stroke types with a high mortality and significant disability burden. Inhibiting ferroptosis after ICH will effectively treat ICH and improve prognosis. Esculetin is the main active ingredient of the Chinese medicine Cortex Fraxini, which has neuroprotective effects. 7-methylguanosine (m7G) methylation is a common RNA modification that regulates mRNA stability and translation, playing an important role in neural development and function, as well as in ferroptosis-related pathways. In this study, we used hemin-induced PC12 cells to investigate the impact of esculetin on ferroptosis and the involvement of m7G modification. Cell viability was measured by cell counting kit-8. Cell death was evaluated by measuring the levels of LDH releas2025-01-01T00:00:00Z2025 Aug2026-05-08T06:46:43.553Z2026-04-07T23:30:32.811ZS-EPMC123739904084688910.1038/s41598-025-16869-7