biostudies-literatureUnknown12Panda CA healthy diet helps transition individuals towards numerous long-term metabolic benefits but is challenging to maintain. Supplementation with additional whole food-based concentrates and herbs may support this shift by enhancing metabolic resilience. This randomized, controlled, crossover study evaluated a 21-day purification program emphasizing plant foods and whole food supplementation on metabolic detoxification, oxidative stress, and gut microbiota composition. Participants followed a plant-based diet for 7 days, with lean proteins introduced from days 8-21. Whole food supplements rich in polyphenols, glucosinolates, and prebiotic fibers were included to support metabolic and gastrointestinal function. The intervention group exhibited 9% (<i>p</i> < 0.0001) reduction in ROS-associatedFrontiers in nutrition1621709https://www.ebi.ac.uk/biostudies/studies/S-EPMC12376428biostudies-literatureMetabolic reset purification program improves antioxidant balance and gut microbiome in individuals transitioning to a healthier diet.PMC12376428Kruse RMetzger BPourafshar SGudavalli MWilliams KKomarnytsky SNance BPanda CfalseMetabolic reset purification program improves antioxidant balance and gut microbiome in individuals transitioning to a healthier diet.A healthy diet helps transition individuals towards numerous long-term metabolic benefits but is challenging to maintain. Supplementation with additional whole food-based concentrates and herbs may support this shift by enhancing metabolic resilience. This randomized, controlled, crossover study evaluated a 21-day purification program emphasizing plant foods and whole food supplementation on metabolic detoxification, oxidative stress, and gut microbiota composition. Participants followed a plant-based diet for 7 days, with lean proteins introduced from days 8-21. Whole food supplements rich in polyphenols, glucosinolates, and prebiotic fibers were included to support metabolic and gastrointestinal function. The intervention group exhibited 9% (<i>p</i> < 0.0001) reduction in ROS-associated2025-01-01T00:00:00Z20252026-07-15T09:45:55.697Z2026-07-02T03:12:13.353ZS-EPMC123764284086047910.3389/fnut.2025.1621709