{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhou F"],"funding":["STI2030-Major Project","National Key R&D Program of China","2022 Shanghai \"Science and Technology Innovation Action Plan\" Fundamental Research Project","National Natural Science Foundation of China","National Key Research and Development Program of China"],"pagination":["e04468"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12376569"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(30)"],"pubmed_abstract":["Achieving detailed neuronal structural information in large-volume brain tissue has been a longstanding challenge in human brain imaging. A key obstacle arises from the trade-off between staining efficiency and tissue autolysis. Traditional Golgi staining, typically conducted at room temperature or 37 °C to optimize staining efficiency, leads to rapid autolysis of brain tissue, resulting in the loss of fine structural details. Here, a near-freezing temperature (NFT) staining strategy in post-mortem frozen (PMF) human brain samples are presented, using a mercury chloride-based method under ice-water bath conditions. In contrast to the 37 °C Golgi staining, this NFT-based method significantly reduces tissue autolysis, preserving fine neuronal structures. Notably, neuronal counts in the same "],"journal":["Advanced science (Weinheim, Baden-Wurttemberg, Germany)"],"pubmed_title":["Near-Freezing-Temperature Golgi Neuronal Staining for X-ray Imaging of Human Brain."],"pmcid":["PMC12376569"],"funding_grant_id":["2021ZD0201100","2022YFA1603600","22325406","T2188102","22393934","21991134","22JC1401203"],"pubmed_authors":["Li J","Zhang J","Zhu Y","Li Q","Yan X","Wang L","Ma C","Hu J","Tang Q","Zhang Y","Zhou F","Cai X","Fan C"],"additional_accession":[]},"is_claimable":false,"name":"Near-Freezing-Temperature Golgi Neuronal Staining for X-ray Imaging of Human Brain.","description":"Achieving detailed neuronal structural information in large-volume brain tissue has been a longstanding challenge in human brain imaging. A key obstacle arises from the trade-off between staining efficiency and tissue autolysis. Traditional Golgi staining, typically conducted at room temperature or 37 °C to optimize staining efficiency, leads to rapid autolysis of brain tissue, resulting in the loss of fine structural details. Here, a near-freezing temperature (NFT) staining strategy in post-mortem frozen (PMF) human brain samples are presented, using a mercury chloride-based method under ice-water bath conditions. In contrast to the 37 °C Golgi staining, this NFT-based method significantly reduces tissue autolysis, preserving fine neuronal structures. Notably, neuronal counts in the same ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-05-09T19:08:06.951Z","creation":"2026-04-08T01:09:49.655Z"},"accession":"S-EPMC12376569","cross_references":{"pubmed":["40434052"],"doi":["10.1002/advs.202504468"]}}