{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Xiao B"],"funding":["National Natural Science Foundation of China","National Key Research and Development Program","National Key Research and Development Program of China"],"pagination":["e04497"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12376627"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(31)"],"pubmed_abstract":["Accurate, real-time detection of neurotransmitters is crucial for elucidating the mechanisms of brain function and tracking the progression of neurological diseases. Biosensors usually face poor reusability induced by difficulties in probe-target separation, hindering their application for continuous monitoring. In this work, a semiconducting carbon nanotube (CNT) film field-effect transistor (FET) biosensor is developed using pH-sensitive aptamers as probes to capture targets. Through tuning the potential of on-chip palladium electrodes, the biosensor facilitates in situ pH modulation and recovery of the sensor interface, which enables an electrically resettable biosensor. The fabricated sensor demonstrates exceptional sensitivity (with femtomolar-level detection limits), high selectivity (specific responses are 20 times stronger than non-specific ones), and excellent reusability (over ten reuse cycles). Furthermore, in vitro detection demonstrates that the biosensor arrays, incorporating regional modifications and pH-sensitive probes, enable the simultaneous detection of several neurotransmitters, including dopamine, serotonin, histamine, and glutamate in complex biological samples. The combination of microfluidic techniques further lowers non-specific adsorption and cross-reactivity, ensuring reliable, repeatable real-time detection. The resettable CNT FET biosensor array holds significant promise for advancing the monitoring of neurotransmitter dynamics, serving as a powerful tool for the early diagnosis and management of neurological disorders."],"journal":["Advanced science (Weinheim, Baden-Wurttemberg, Germany)"],"pubmed_title":["In Situ Electrically Resettable Field-Effect Transistor Biosensors for Continuous and Multiplexed Neurotransmitter Detection."],"pmcid":["PMC12376627"],"funding_grant_id":["2022YFB3204402","62174007","2022YFB4401601","62225101"],"pubmed_authors":["Xiao M","Cao X","Li T","He J","Zhang Z","Zhang Y","Xiao B"],"additional_accession":[]},"is_claimable":false,"name":"In Situ Electrically Resettable Field-Effect Transistor Biosensors for Continuous and Multiplexed Neurotransmitter Detection.","description":"Accurate, real-time detection of neurotransmitters is crucial for elucidating the mechanisms of brain function and tracking the progression of neurological diseases. Biosensors usually face poor reusability induced by difficulties in probe-target separation, hindering their application for continuous monitoring. In this work, a semiconducting carbon nanotube (CNT) film field-effect transistor (FET) biosensor is developed using pH-sensitive aptamers as probes to capture targets. Through tuning the potential of on-chip palladium electrodes, the biosensor facilitates in situ pH modulation and recovery of the sensor interface, which enables an electrically resettable biosensor. The fabricated sensor demonstrates exceptional sensitivity (with femtomolar-level detection limits), high selectivity (specific responses are 20 times stronger than non-specific ones), and excellent reusability (over ten reuse cycles). Furthermore, in vitro detection demonstrates that the biosensor arrays, incorporating regional modifications and pH-sensitive probes, enable the simultaneous detection of several neurotransmitters, including dopamine, serotonin, histamine, and glutamate in complex biological samples. The combination of microfluidic techniques further lowers non-specific adsorption and cross-reactivity, ensuring reliable, repeatable real-time detection. The resettable CNT FET biosensor array holds significant promise for advancing the monitoring of neurotransmitter dynamics, serving as a powerful tool for the early diagnosis and management of neurological disorders.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-05-09T17:48:03.706Z","creation":"2026-04-08T01:07:29.692Z"},"accession":"S-EPMC12376627","cross_references":{"pubmed":["40433802"],"doi":["10.1002/advs.202504497"]}}