biostudies-literatureXiao BNational Natural Science Foundation of ChinaNational Key Research and Development ProgramNational Key Research and Development Program of Chinae04497https://www.ebi.ac.uk/biostudies/studies/S-EPMC12376627biostudies-literatureUnknown12(31)Accurate, real-time detection of neurotransmitters is crucial for elucidating the mechanisms of brain function and tracking the progression of neurological diseases. Biosensors usually face poor reusability induced by difficulties in probe-target separation, hindering their application for continuous monitoring. In this work, a semiconducting carbon nanotube (CNT) film field-effect transistor (FET) biosensor is developed using pH-sensitive aptamers as probes to capture targets. Through tuning the potential of on-chip palladium electrodes, the biosensor facilitates in situ pH modulation and recovery of the sensor interface, which enables an electrically resettable biosensor. The fabricated sensor demonstrates exceptional sensitivity (with femtomolar-level detection limits), high selectivity (specific responses are 20 times stronger than non-specific ones), and excellent reusability (over ten reuse cycles). Furthermore, in vitro detection demonstrates that the biosensor arrays, incorporating regional modifications and pH-sensitive probes, enable the simultaneous detection of several neurotransmitters, including dopamine, serotonin, histamine, and glutamate in complex biological samples. The combination of microfluidic techniques further lowers non-specific adsorption and cross-reactivity, ensuring reliable, repeatable real-time detection. The resettable CNT FET biosensor array holds significant promise for advancing the monitoring of neurotransmitter dynamics, serving as a powerful tool for the early diagnosis and management of neurological disorders.Advanced science (Weinheim, Baden-Wurttemberg, Germany)In Situ Electrically Resettable Field-Effect Transistor Biosensors for Continuous and Multiplexed Neurotransmitter Detection.PMC123766272022YFB3204402621740072022YFB440160162225101Xiao MCao XLi THe JZhang ZZhang YXiao BfalseIn Situ Electrically Resettable Field-Effect Transistor Biosensors for Continuous and Multiplexed Neurotransmitter Detection.Accurate, real-time detection of neurotransmitters is crucial for elucidating the mechanisms of brain function and tracking the progression of neurological diseases. Biosensors usually face poor reusability induced by difficulties in probe-target separation, hindering their application for continuous monitoring. In this work, a semiconducting carbon nanotube (CNT) film field-effect transistor (FET) biosensor is developed using pH-sensitive aptamers as probes to capture targets. Through tuning the potential of on-chip palladium electrodes, the biosensor facilitates in situ pH modulation and recovery of the sensor interface, which enables an electrically resettable biosensor. The fabricated sensor demonstrates exceptional sensitivity (with femtomolar-level detection limits), high selectivity (specific responses are 20 times stronger than non-specific ones), and excellent reusability (over ten reuse cycles). Furthermore, in vitro detection demonstrates that the biosensor arrays, incorporating regional modifications and pH-sensitive probes, enable the simultaneous detection of several neurotransmitters, including dopamine, serotonin, histamine, and glutamate in complex biological samples. The combination of microfluidic techniques further lowers non-specific adsorption and cross-reactivity, ensuring reliable, repeatable real-time detection. The resettable CNT FET biosensor array holds significant promise for advancing the monitoring of neurotransmitter dynamics, serving as a powerful tool for the early diagnosis and management of neurological disorders.2025-01-01T00:00:00Z2025 Aug2026-05-09T17:48:03.706Z2026-04-08T01:07:29.692ZS-EPMC123766274043380210.1002/advs.202504497